Abstract
There was developed and certified the analytical method for the determination of thiodiacetic acid in the urine in the range of from 0.1 to 10µg/cm3. The sample preparation is based on the execution of derivatization of methanol in the presence of boron trifluoride (10 wt%) followed by liquid-liquid microextraction with dimethyl ester the acid. Derivatization reaction was carried out for 15 min at 80°C. Gas chromatographic analysis was performed on a capillary column HP-5ms under the temperature gradient and splitless modes. Mass spectrometric detection occurred during the registration of selective ions (SIM). Identification of derivatives of thiodiacetic acid on the mass chromatograms was carried out by the absolute retention time (10.36 min) and the ratio of peak intensities of detected ions. The random error in the determination of component and the accuracy of the expanded uncertainty does not exceed 2 and 25%, respectively. There was made a comparative evaluation of the content of thiodiacetic acid in the urine of workers of main shops production of PVC with persons of the control group, not involved in the production. The results can be used for the identification of the fact of the exposure to toxic substances and assessment of the level of exposure and biomonitoring, as a component of health interventions for the prevention of occupational and production-related diseases.
