Email this article DETERMINATION OF RICIN IN ENVIRONMENTAL SAMPLES USING BIOASSAY
- Authors: Rogovskaya N.Y.1, Gorbunov A.Y.1, Dubrovskii Y.A.1, Khlebnikova N.S.1, Babakov V.N.1
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Affiliations:
- Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical Biological Agency
- Issue: No 5 (2020)
- Pages: 43-49
- Section: Articles
- Published: 05.11.2020
- URL: https://vestnik.nvsu.ru/0869-7922/article/view/641261
- DOI: https://doi.org/10.36946/0869-7922-2020-5-43-49
- ID: 641261
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Abstract
A bioassay of ricin toxicity in environmental samples using real-time cell index monitoring is proposed. The halfmaximal inhibitory concentration (IC50) of ricin was estimated at 6,7 ng/ml for human hepatoma HepaRG cells proliferation. The antibodies to A- and B-subunits in HepaRG cell media lead to cytoprotective and antiapoptotic effects against the cytotoxicity of ricin. The antibodies neutralised activation of JNK kinase (phosphorylated at Thr183/Tyr185) and prevented accumulation of the active forms of caspase 8 (hydrolysed to Asp384) and caspase 9 (hydrolysed to Asp315) induced by ricin in HepaRG cells. The tested antibodies also prevented a decrease in the intracellular levels of the active forms of Akt 1 kinase (phosphorylated at Ser473) and transcription factor p53 (phosphorylated at Ser46) caused by ricin. The bioassay with antibodies can be considered as a specific method for identifying the toxin in environmental samples.
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About the authors
N. Yu. Rogovskaya
Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical Biological Agency
Author for correspondence.
Email: niigpech@rihophe.ru
Rogovskaya Nadezhda Yur′evna
188663, Leningrad region
Russian FederationA. Yu. Gorbunov
Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical Biological Agency
Email: niigpech@rihophe.ru
Gorbunov Aleksandr Yur′evich
188663, Leningrad region
Russian FederationYa. A. Dubrovskii
Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical Biological Agency
Email: niigpech@rihophe.ru
Dubrovskii Yaroslav Aleksandrovich
188663, Leningrad region
Russian FederationN. S. Khlebnikova
Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical Biological Agency
Email: niigpech@rihophe.ru
Khlebnikova Natalia Semenovna
188663, Leningrad region
Russian FederationV. N. Babakov
Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical Biological Agency
Email: babakov@rihophe.ru
Babakov Vladimir Nikolaevich
188663, Leningrad region
Russian FederationReferences
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