CYTOLOGICAL AND BIOCHEMICAL CHARACTERISTICS OF BRONCHOALVEOLAR LAVAGE FLUID IN RATS AFTER INTRATRACHEAL INSTILLATION OF COPPER OXIDE NANO-SCALE PARTICLES

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Abstract

Stable water suspensions of copper oxide particles with the mean diameter of 20 nm and copper oxide-copper ones with the mean diameter of 340 nm were used to assess the rats pulmonary phagocytosis response to a single intratracheal instillation of 0.5 mg(1 ml) of these suspensions followed by the bronchoalveolar lavage after 24 hours with the help of optical, transmission electron, and semi-contact atomic force microscopy and a number of biochemical indices measured in the bronchoalveolar lavage fluid. It was found out that the two fractions produce an expressed toxic effect on lungs but nanoparticles appear significantly more toxic than sub-micron particles greater than 100 nm in size while the former evokes a more pronounced defense recruitment of alveolar macrophages and especially of neutrophil leukocytes at a higher phagocytosis activity of these cells. Results obtained compared to literature data show that a high cytotoxic action of nanometer copper oxide particles may be caused both by a copper ions intracellular release at solubilization and direct contact of persistent particles internalized by phagocytes with cell organelles (primarily, mitochondria) and their injuryas well.

About the authors

Larisa Ivanovna Privalova

Ekaterinburg Medical Research Center for Prophylaxis and Health Protection of Industrial Workers

Author for correspondence.
Email: noemail@neicon.ru

Доктор медицинских наук, профессор, заведующая лабораторией научных основ биопрофилактики ФБУН  МНЦПОЗРПП. 620014, г. Екатеринбург

e-mail: privalovali@yahoo.com

Russian Federation

Boris Aleksandrovich Katsnelson

Ekaterinburg Medical Research Center for Prophylaxis and Health Protection of Industrial Workers

Email: noemail@neicon.ru

Доктор медицинских наук, профессор, заведующий отделом токсикологии и биопрофилактики ФБУН ЕМНЦПОЗРПП. 620014, г. Екатеринбург

e-mail: bkaznelson@etel.ru

Russian Federation

Nadezhda Vladimirovna Loginova

Ekaterinburg Medical Research Center for Prophylaxis and Health Protection of Industrial Workers

Email: noemail@neicon.ru

Научный сотрудник лаборатории научных основа биопрофилактики ФБУН ЕМНЦПОЗРПП. 620142, г. Екатеринбург,

e-mail: loginovan@ymrc.ru

Russian Federation

Vladimir Borisovich Gurvich

Ekaterinburg Medical Research Center for Prophylaxis and Health Protection of Industrial Workers

Email: noemail@neicon.ru

Доктор медицинских наук, директор ФБУН ЕМНЦПОЗРПП; 620014, г. Екатеринбург.

e-mail: gurvich@ymrc.ru

Russian Federation

Vladimir Yakovlevich Shur

The Centre for collective use «Modern Nanotechnologies», Ural Federal University

Email: noemail@neicon.ru

Доктор физико-математических наук, профессор, директор Центра коллективного пользования «Современные нанотехнологии», 620000, г. Екатеринбург

e-mail: vladimir.shur@usu.ru

Russian Federation

Yakov Borisovich Beikin

The City Clinical Diagnostics Centre

Email: noemail@neicon.ru

Доктор медицинских наук, профессор, директор МУ «Клинико-диагностический центр», 620142, г. Екатеринбург

e-mail: kdc_boss@mail.ru

Russian Federation

Marina Petrovna Sutunkova

Ekaterinburg Medical Research Center for Prophylaxis and Health Protection of Industrial Workers

Email: noemail@neicon.ru

Кандидат медицинских наук, старший научный сотрудник отдела токсикологии и биопрофилактики ФБУН ЕМНЦПОЗРПП, 620014, г. Екатеринбург

e-mail: sutunkova@ymrc.ru

Russian Federation

Ilzira Amirovna Minigalieva

Ekaterinburg Medical Research Center for Prophylaxis and Health Protection of Industrial Workers

Email: noemail@neicon.ru

Кандидат биологических наук, старший научный сотрудник отдела токсикологии и биопрофилактики ФБУН ЕМНЦПОЗРПП, 620014, г. Екатеринбург

e-mail: ilzira-minigalieva@yandex.ru

Russian Federation

Ekaterina Vladimirovna Shishkina

The Centre for collective use «Modern Nanotechnologies», Ural Federal University

Email: noemail@neicon.ru

Кандидат физико-математических наук, старший научный сотрудник Центра коллективного пользования «Современные нано технологии», 620000, г. Екатеринбург

e-mail: ekaterina.shishkina@labfer.usu.ru

Russian Federation

Svetlana Vladimirovna Pichugova

The City Clinical Diagnostics Centre

Email: noemail@neicon.ru

Специалист лаборатории электронной микроскопии «Клинико-диагностический центр», 620142, г. Екатеринбург

e-mail: ekb-lem@mail.ru

Russian Federation

Ludmila Gennadevna Tulakina

The City Clinical Diagnostics Centre

Email: noemail@neicon.ru

Кандидат медицинских наук, заведующий лабораторией электронной микроскопии «Клинико-диагностический центр», 620142, г. Екатеринбург

e-mail: tulakina@inbox.ru

Russian Federation

Svetlana Valentinovna Beljayeva

The City Clinical Diagnostics Centre

Email: noemail@neicon.ru

Кандидат биологических наук, заведующий биохимической лабораторией МУ «Клинико-диагностический центр», 620142, г. Екатеринбург

e-mail: kdc_boss@mail.ru

Russian Federation

Vadim Olegovich Rusakov

Ekaterinburg Medical Research Center for Prophylaxis and Health Protection of Industrial Workers

Email: noemail@neicon.ru

Аспирант ФБУН ЕМНЦ ПОЗРПП, 620014, г. Екатеринбург

e-mail: ruzakov_vo@66.rospotrebnadzor.ru

Russian Federation

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Copyright (c) 2014 Privalova L.I., Katsnelson B.A., Loginova N.V., Gurvich V.B., Shur V.Y., Beikin Y.B., Sutunkova M.P., Minigalieva I.A., Shishkina E.V., Pichugova S.V., Tulakina L.G., Beljayeva S.V., Rusakov V.O.


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