Enviar artigo por via de e-mail LOWER AIRWAYS RESPONSE IN RATS TO A SINGLE OR COMBINED INTRATRACHEAL INSTILLATION OF MANGANESE AND NICKEL NANOPARTICLES AND ITS ATTENUATION WITH A BIO-PROTECTIVE PRE-TREATMENT
- Autores: Katsnelson B.A.1, Minigalieva I.A.1, Privalova L.I.1, Sutunkova M.P.1, Gurvich V.B.1, Shur V.Y.2, Shishkina E.V.2, Varaksin A.N.3, Panov V.G.3
-
Afiliações:
- Federal Budgetary Science Institution«Ekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers»
- Centre for Shared Use «Modern Nanotechnologies» of the Ural Federal University
- Institute of Industrial Ecology, Ural Branch of the Russian Academy of Sciences
- Edição: Nº 6 (2014)
- Páginas: 8-14
- Seção: Articles
- ##submission.datePublished##: 22.12.2014
- URL: https://vestnik.nvsu.ru/0869-7922/article/view/641376
- DOI: https://doi.org/10.15690/vramn543
- ID: 641376
Citar
Texto integral
Resumo
Nickel(II) oxide and multiple manganese species [manganese(II, III) oxide included], may be jointly present in welding fumes submicron fraction. Using laser ablation of 99.99% pure metallic plates in deionized water, suspensions of NiO and Mn3O4 nanoparticles with mean (±σ) diameters 30±12 nm and 32±10 nm respectively were created. Both suspensions were instilled intratracheally to rats, one immediately after another in alternating sequence or separately (another instillation being with water). The broncho alveolar lavage fluid (BALF) was obtained 24 h after. The increase in total BALF cells, alveolar macrophages (AM) and neutrophil leukocytes (NL) counts and especially in the NL/AM ratio (which is a reliable сomparison index of instilled particles cytotoxicity) was significantly higher at exposure to NiO than to Mn3O4. Semi-contact atomic force microscopy of cell surface topography demonstrated active endocytosis of NiO and Mn3O4 nanoparticles by pulmonary phagocytes. The higher pulmonary cytotoxicity of NiO as compared with Mn3O4 nanoparticles was also shown by increase in some lysosomal enzyme activities. Mathematical analysis revealed a sub-additive combined action of NiO and Mn3O4, judging by cellular indices, while judging by different biochemical indices additivity, sub-additivity or synergism were shown. In rats fed glutamate, glycine, acetyl cysteine, iodide and a Se-containing multivitamin preparation orally during 4 weeks before exposure to NiO+Mn3O4 (0.25 mg each), the latter evoked a significantly weaker NL recruitment than in rats so exposed without pretreatment.
Sobre autores
Boris Katsnelson
Federal Budgetary Science Institution«Ekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers»
Autor responsável pela correspondência
Email: noemail@neicon.ru
Доктор медицинских наук, профессор, заведующий отделом токсикологии и биопрофилактики ФБУН ЕМНЦПОЗРПП, 620014, г. Екатеринбург
e-mail: bkaznelson@etel.ru
RússiaIlzira Minigalieva
Federal Budgetary Science Institution«Ekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers»
Email: noemail@neicon.ru
Кандидат биологических наук, старший научный сотрудник отдела токсикологии и биопрофилактики ФБУН ЕМНЦПОЗРПП, 620014, г. Екатеринбург
e-mail: ilzira-minigalieva@yandex.ru
Rússia
Larisa Privalova
Federal Budgetary Science Institution«Ekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers»
Email: noemail@neicon.ru
Доктор медицинских наук, профессор, заведующая лабораторией научных основ биопрофилактики ФБУН ЕМНЦПОЗРПП, 620014, г. Екатеринбург
e-mail: privalovali@yahoo.com
RússiaMarina Sutunkova
Federal Budgetary Science Institution«Ekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers»
Email: noemail@neicon.ru
Кандидат медицинских наук, старший научный сотрудник отдела токсикологии и биопрофилактики ФБУН ЕМНЦПОЗРПП, 620014, г. Екатеринбург
e-mail: sutunkova@ymrc.ru
RússiaVladimir Gurvich
Federal Budgetary Science Institution«Ekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers»
Email: noemail@neicon.ru
Доктор медицинских наук, директор ФБУН ЕМНЦПОЗРПП, 620014, г. Екатеринбург
e-mail: gurvich@ymrc.ru
RússiaVladimir Shur
Centre for Shared Use «Modern Nanotechnologies» of the Ural Federal University
Email: noemail@neicon.ru
Доктор физико-математических наук, профессор, директор Центра коллективного пользования «Современные нанотехнологии», 620000, г. Екатеринбург
e-mail: vladimir.shur@usu.ru
RússiaEkaterina Shishkina
Centre for Shared Use «Modern Nanotechnologies» of the Ural Federal University
Email: noemail@neicon.ru
Кандидат физико-математических наук, старший научный сотрудник Центра коллективного пользования «Современные нанотехнологии», 620000, г. Екатеринбург
e-mail: ekaterina.shishkina@labfer.usu.ru
RússiaAnatoliy Varaksin
Institute of Industrial Ecology, Ural Branch of the Russian Academy of Sciences
Email: noemail@neicon.ru
Доктор физико-математических наук, профессор, главный научный сотрудник лаборатории математического моделирования в экологии и медицине Института промышленной экологии, нанотехнологии, 620990, г. Екатеринбург,
e-mail: varaksin@ecko.uran.ru
RússiaVladimir Panov
Institute of Industrial Ecology, Ural Branch of the Russian Academy of Sciences
Email: noemail@neicon.ru
Кандидат физико-математических наук, зав. лабораторией математического моделирования в экологии и медицине Института промышленной экологии, нанотехнологии, 620990, г. Екатеринбург
e-mail: panov@ecko.uran.ru
RússiaBibliografia
- Katsnelson B.A. Combined action of chemicals. In: Kurlyandsky BA, Filov VA (eds.). Moscow: «Meditsina» Publishers, 2002: 497-520 (in Russian).
- Varaksin A.N., Katsnelson B.A., Panov V.G., Privalova L.I., Kireyeva E.P., Valamina I.E., Beresneva O.Yu. Some considerations concerning the theory of combined toxicity: a case study of subchronic experimental intoxication with cadmium and lead. Food Chem. Toxicol. 2014; 64: 144-156.
- Keane M., Stone S., Chen B. Welding fumes from stainless steel gas metal arc processes contain multiple manganese chemical species. J. Environ. Monit. 2010; 12: 1130-1140.
- Taube F. Manganese in occupational arc welding fumes - aspects on physiochemical properties, with focus on solubility. Ann. Occup. Hyg. 2012; 57: 6-25.
- Andujar P., Simon-Deckers A., Galateau-Salle F., Fayard B., Beaune G., Clin B., Billon-Galland M-A., Durupthy O., Pairon J-C., Doucet J., et al. Role of metal oxide nanoparticles in histopathological changes observed in the lung of welders. Part. Fibre Toxicol. 2014; 11: 23.
- Katsnelson B.A., Privalova L.I., Kuzmin S.V., Degtyareva T.D., Sutunkova M.P., Yeremenko O.S., Minigalieva I.A., Kireyeva E.P., Khodos M.Y., Kozitsina A.N., et al. Some peculiarities of pulmonary clearance mechanisms in rats after intratracheal instillation of magnetite (Fe3O4) suspensions with different particle sizes in the nanometer and micrometer ranges: Are we defenseless against nanoparticles? Int. J. Occup. Environ. Health. 2010; 16: 508-524.
- Katsnelson B.A., Privalova L.I., Degtyareva T.D., Sutunkova M.P., Yeremenko O.S., Minigalieva I.A., Kireyeva E.P., Kozitsina A.N., Malakhova N.A., Glazyrina J.A., et al. Experimental estimates of the toxicity of iron oxide Fe3O4 (magnetite) nanoparticles. Cent. Eur. J. Occup. Environ. Med. 2010; 16: 47-63.
- Katsnelson B.A., Privalova L.I., Gurvich V.B., Makeyev O.H., Shur V.Y., Beikin J.B., Sutunkova M.P., Kireyeva E.P., Minigalieva I.A., Loginova N.V., et al. Comparative in vivo assessment of some adverse bio-effects of equidimensional gold and silver nanoparticles and the attenuation of nanosilver’s effects with a complex of innocuous bioprotectors. Int. J. Mol. Sci. 2013; 14: 2449-2483.
- Privalova L.I., Katsnelson B.A., Loginova N.V., Gurvich V.B., Shur V.Y., Beikin J.B.et al. Cytological and biochemical characteristics of bronchoalveolar lavage fluid in rats after intratracheal instillation of nano-scale copper oxide particles. Toxic. Vestnik. 2014; 5 (in Russian).
- Gessner P.K. Isobolographic analysis of interactions: an update on applications and utility. Toxicology. 1995; 105: 161-179.
- Privalova L.I., Katsnelson B.A., Osipenko A.B., Yushkov B.H., Babushkina L.G. Response of a phagocyte cell system to products of macrophage breakdown as a probable mechanism of alveolar phagocytosis adaptation to deposition of particles of different cytotoxicity. Environm. Health Perspect.1980; 35: 205-218.
- Katsnelson B.A., Privalova L.I. Recruitment of phagocytizing cells into the respiratory tract as a response to the cytotoxic action of deposited particles. Environ. Health Perspect.1984; 55: 313-325.
- Gennings C. On testing for drug/ chemical interaction: definitions and inference. J. Biopharm. Stat. 2000; 10(4): 457-467.
- Katsnelson D.A., Balesin S.L., Davydova V.I., Neisvestnova E.M., Privalova L.I., Konysheva L.I. General patterns, experimental and mathematical methods of assessing combined toxicity of metals. In: «Proceedings of the plenary meetings of executive boards of the All-Union and Armenian Toxicological Societies dedicated to the problem of combined action of chemicals on the organism», Yerevan, 1989: 14-17 (in Russian).
- Donaldson K., Bolton R., Jones E.A., Brown G.M., Robertson M.D.,Slight J., Cowie H., Davis J.M.G. Kinetics of the bronchoalveolar leucocyte response in rats during exposure to equal airborne mass concentrations of quartz, chrysotile asbestos, or titanium dioxide. Thorax. 1988;43: 525-533.
- Zhang Q., Yukinori K., Sato K., Nakakuki K., Koyahama N., Donaldson K. Differences in the extent of inflammation caused by intratracheal exposure to three ultrafine metals: role of free radicals. J. Toxicol. Environ. Health. 1998; 53: 423-438.
- Zhang Q., Yukinori K., Zhu X., Sato K., Mo Y., Kluz T., Donaldson K. Comparative toxicity of standard nickel and ultrafine nickel after intratracheal instillation. J. Occip. Health. 2003; 45: 23-30.
Arquivos suplementares
