Some aspects of metal oxide nanoparticles toxicity assessment on cell cultures as exemplified by NiO and Mn3O4

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Comparative and combined damaging actions of NiO and Mn3O4 anoparticles were estimated on cultures of different established human cell lines. It was found out that the addition of the fetal bovine serum (FBS) to the culture media ,used in the investigation, renders NiO-NPs and, to even a greater extent, Mn3O4-NPs exponentially soluble while without FBS their dissolution was extremely low. Along with it, sedimentation of those MeO-NPs caused by their aggregation noticeably slowed down in the presence of the same FBS. The dependence of cell damage on the MeO-NPs concentration was found out, at a higher cytotoxicity of Mn3O4-NP as compared to NiO-NP. Thus, comparative assessment of NPs non-specific toxicity previously obtained in animal experiments was reproduced in the «in vitro» tests. However, with respect to manganese-specific brain damage «in vivo» discovered previously in sub-chronic intoxication with the same MeO-NPs, the present «in vitro» experiment on neurons only showed a certain enhancing effect of Mn3O4-NP on the action of NiO-NP, but the role of NiO-NP in the combination prevailed.

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I. Minigalieva

Medical Research Center for Prophylaxis and Health Protection in Industrial Workers

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T. Bushueva

Medical Research Center for Prophylaxis and Health Protection in Industrial Workers

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V. Panov

Institute of Industrial Ecology, the Urals Branch of the Russian Academy of Sciences

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A. Varaksin

Institute of Industrial Ecology, the Urals Branch of the Russian Academy of Sciences

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V. Shur

School of Natural Sciences and Mathematics, the Ural Federal University

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E. Shishkina

School of Natural Sciences and Mathematics, the Ural Federal University

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V. Gurviсh

Medical Research Center for Prophylaxis and Health Protection in Industrial Workers

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B. Katsnelson

Medical Research Center for Prophylaxis and Health Protection in Industrial Workers

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版权所有 © Minigalieva I.А., Bushueva T.V., Panov V.G., Varaksin A.N., Shur V.Y., Shishkina E.V., Gurviсh V.B., Katsnelson B.A., 2017


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