EFFECT OF ARYL HYDROCARBON RECEPTOR AGONISTS AND LIPOPOLYSACCHARIDE ON BENZO(A)PYRENE GENOTOXICITY MARKERS

封面

如何引用文章

全文:

详细

The effect of aryl hydrocarbon receptor agonists (FICZ and ITE), as well as lipopolysaccharide under the toxic action of benzo(a)pyrene in HepaRG human hepatoma cells was evaluated. Active forms of the key stress-activated kinase cascades and DNA repair system proteins were used as markers of the genotoxic action of benzo(a)pyrene. A mixture of lipopolysaccharide with benzo(a)pyrene increases benzo(a)pyrene cytotoxicity and reduces the activation of DNA repair system proteins below the control level. Aryl hydrocarbon receptor agonists (FICZ and ITE) exhibit a cytoprotective effect against benzo(a) pyrene, enhance Akt1 kinase activation, and downregulate activation of the p53 protein and Chk1 and Chk2 checkpoint kinases. Thus, FICZ and ITE reduce the genotoxicity of benzo(a)pyrene.

作者简介

V. Babakov

Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical Biological Agency

编辑信件的主要联系方式.
Email: babakov@rihophe.ru

Babakov Vladimir Nikolaevich

Saint Petersburg

俄罗斯联邦

N. Rogovskaya

Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical Biological Agency

Email: fake@neicon.ru

Rogovskaya Nadezhda Yurievna

Saint Petersburg

俄罗斯联邦

I. Kurdyukov

Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical Biological Agency

Email: fake@neicon.ru

Kurdyukov Ivan Dmitrievich

Saint Petersburg

俄罗斯联邦

P. Beltyukov

Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical Biological Agency

Email: fake@neicon.ru

Beltyukov Petr Petrovich

Saint Petersburg

俄罗斯联邦

S. Dulov

Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical Biological Agency

Email: fake@neicon.ru

Dulov Sergei Anatolievich

Saint Petersburg

俄罗斯联邦

A. Radilov

Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical Biological Agency

Email: radilov@rihophe.ru

Radilov Andrei Stanislavovich

Saint Petersburg

俄罗斯联邦

参考

  1. Coates P.J., Lorimore S.A., Wright E.G. Cell and tissue responses to genotoxic stress. J Pathol. 2005; 205(2):221-35.
  2. Shimada T. Inhibition of carcinogen-activating cytochrome P450 enzymes by xenobiotic chemicals in relation to antimutagenicity and anticarcinogenicity. Toxicol Res. 2017; 33(2):79-96.
  3. Siddens L.K., Larkin A., Krueger S.K., et al. Polycyclic aromatic hydrocarbons as skin carcinogens: comparison of benzo[a]pyrene, dibenzo[def,p]chrysene and three environmental mixtures in the FVB/N mouse. Toxicol. Appl. Pharmacol. 2012; 264(3): 377-86.
  4. Niida H., Nakanishi M. DNA damage checkpoints in mammals. Mutagenesis. 2006; 21(1):3-9.
  5. Babakov V.N., Rogovskaya N.Yu., Kurdyukov I.D., Beltyukov P.P., Dulov S.A., Radilov A.S. The effect of benzo(a)-pyrene and the endogenous agonist of the aryl hydrocarbon receptor FICZ on the activation of the transcription factor NF-kB and the cytokine secretion in human hepatoma HepaRG cells. Medicine of Extreme Situations. 2018; 20(3): 432-438 (in Russian).
  6. Lubberstedt M., Muller-Vieira U., Mayer M., Biemel K.M., Knospel F., Knobeloch D., et al. HepaRG human hepatic cell line utility as a surrogate for primary human hepatocytes in drug metabolism assessment in vitro. J. Pharmacol. Toxicol. Methods. 2011; 63(1): 59-68.
  7. Lin T., Yang M.S. Benzo[a]pyrene-induced necrosis in the HepG2 cells via PARP-1 activation and NAD+ depletion. Toxicology. 2008; 245(1–2): 147-153.
  8. Rogovskaya N.Yu.,Babakov V.N. Apoptosis markers are molecular targets of sulfure mustard toxic action. Toksikologicheskii vestnik (Toxicological Review). 2017; (5): 28-34 (in Russian).
  9. Volkov M.S., Kobliakov V.A. Activation of transcription factor NF-kappaB by carcinogenic polycyclic aromatic hydrocarbons. Tsitologiia. 2011; 53(5):418-22. (In Russian).
  10. Smerdova L., Neca J., Svobodova J., Topinka J., Schmuczerova J., Kozubik A., et al. Inflammatory mediators accelerate metabolism of benzo[a]pyrene in rat alveolar type II cells: the role of enhanced cytochrome P450 1B1 expression. Toxicology.2013; 314: 30–38.
  11. Uno S., Makishima M. Benzo[a]pyrene toxicity and inflammatory disease. Curr. Rheumatol. Rev. 2009; 5: 266–271.
  12. Arlt V.M., Krais A.M., Godschalk R.W., Riffo-Vasquez Y., Mrizova I., Roufosse et al. Pulmonary inflammation impacts on CYP1A1-mediated respiratory tract DNA damage induced by the carcinogenic air pollutant benzo[a]pyrene. Toxicol. Sci. 2015; 146: 213–225.
  13. Shi Q., Haenen G.R., Maas L., Arlt V.M., Spina D., Vasquez Y.R., et al. Inflammation-associated extracellular beta-glucuronidase alters cellular responses to the chemical carcinogen benzo[a]pyrene. Arch. Toxicol. 2016; 90: 2261–2273.
  14. Shi Q., Fijten R.R., Spina D., Riffo Vasquez Y., Arlt V.M., Godschalk R.W., et al. Altered gene expression profiles in the lungs of benzo[a]pyrene-exposed mice in the presence of lipopolysaccharide-induced pulmonary inflammation. Toxicol Appl Pharmacol. 2017; 336:8-19.
  15. Voronov I., Li K., Tenenbaum H.C., Manolson M.F. Benzo[a]pyrene inhibits osteoclastogenesis by affecting RANKL-induced activation of NF-κB. Biochem. Pharmacol. 2008; 75(10): 2034-2044.
  16. Vorontsova J.E., Cherezov R.O., Kuzin B.A., Simonova O.B. Aryl-hydrocarbon receptor as a potential target for anticancer therapy. Biomed. Khim. 2018; 64(5): 397-415 (in Russian).

补充文件

附件文件
动作
1. JATS XML
下载

版权所有 © Babakov V.N., Rogovskaya N.Y., Kurdyukov I.D., Beltyukov P.P., Dulov S.A., Radilov A.S., 2019


СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС 77 - 81728 от 11 декабря 2013.