电邮这篇文章 Protective influence of the zinc complex of 1-butylvioluric acid on the development of the liver tissue culture in the presence of convulsive poisons
- 作者: Kolbasova T.A.1, Chalisova N.I.1,2, Egozova E.S.3, Ivanova P.N.2, Krasnov K.A.1, Bespalov A.Y.1
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隶属关系:
- Institute of Toxicology of Federal Medico-Biomedical Agency
- Pavlov Institute of Physiology RAS
- Herzen University
- 期: 编号 1 (2023)
- 页面: 18-23
- 栏目: Original articles
- ##submission.datePublished##: 23.02.2023
- URL: https://vestnik.nvsu.ru/0869-7922/article/view/641474
- DOI: https://doi.org/10.47470/0869-7922-2023-31-1-18-23
- ID: 641474
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Introduction. Currently, the actual problem is the search for substances that can be used as drugs for the pharmacological correction of the effects of cytostatic damage by convulsive poisons. It is known that the liver is the first organ to encounter any foreign molecule carried by the portal bloodstream, and it is the most damaged.
The aim of this work was to study the effect of the zinc complex of 1-butylvioluric acid on the development of an organotypic culture of rat liver tissue after poisoning with 2-(dimethylaminomethyl)phenyl ester of dimethylcarbamic acid hydrochloride.
Material and methods. White 3-month-old male Wistar rats were used in the experiment. To study the effects of the test substances, liver tissue was selected and the method of organotypic cultivation was applied. Poisoning with convulsive poisons was modeled using 2-(dimethylaminomethyl)phenyl ester of dimethylcarbamic acid hydrochloride and the zinc complex of 1-butylvioluric acid was used as a drug for pharmacological correction of the effects of liver tissue damage.
Results. The data obtained indicate that under the action of 2-(dimethylaminomethyl) phenyl ester of dimethylcarbamic acid hydrochloride, which simulates the effect of convulsive poisons on liver cells, their cell proliferation is inhibited. It was also found that the zinc complex of 1-butylvioluric acid eliminates the inhibitory effect of 2-(dimethylaminomethyl)phenyl ester of dimethylcarbamic acid hydrochloride on the proliferation of liver tissue culture.
Research limitations. The study was performed on a culture of rat liver cells; to extrapolate data to the whole organism, it is necessary to take into account the data of toxicodynamics and toxicokinetics.
Conclusion. Thus, the data obtained in the experiments prove the effectiveness of the use of the zinc complex of 1-butylvioluric acid as a drug for the pharmacological correction of the consequences of exposure to convulsive poisons, and also create a basis for its further study.
Compliance with ethical standards. Animals were kept in accordance with the requirements of GOST 33044–2014 dated 08/01/2015 “Principles of good laboratory practice”.
Author contribution. All co-authors made an equal contribution to the research and preparation of the article for publication.
Conflict of interests. The authors declare no conflict of interest.
Funding. The study was not sponsored.
Received: September 1, 2022 / Accepted: January 2023 / Published: February 28, 2023
作者简介
Taisiia Kolbasova
Institute of Toxicology of Federal Medico-Biomedical Agency
编辑信件的主要联系方式.
Email: tasia.pcma.le4@mail.ru
ORCID iD: 0000-0003-1646-1760
Candidate of Medical Sciences, Researcher, Laboratory of Biochemical Toxicology and Pharmacology of the «Institute of Toxicology» of Federal Medico-Biomedical Agency, 192019, St. Petersburg, Russian Federation.
e-mail: tasia.pcma.le4@mail.ru
Scopus Author ID: 57209853108
俄罗斯联邦Natalia Chalisova
Institute of Toxicology of Federal Medico-Biomedical Agency; Pavlov Institute of Physiology RAS
Email: ni_chalisova@mail.ru
ORCID iD: 0000-0002-2371-0043
Scopus Author ID: 7006310236
俄罗斯联邦Ekaterina Egozova
Herzen University
Email: ekaterina_egozova@mail.ru
ORCID iD: 0000-0002-0055-3778
Лаборант кафедры анатомии и физиологии человека Федерального государственного бюджетного образовательного учреждения высшего образования «Российский государственный педагогический университет им. А.И. Герцена», (Herzen University), 191186, г. Санкт-Петербург, Российская Федерация.
e-mail: ekaterina_egozova@mail.ru
俄罗斯联邦Polina Ivanova
Pavlov Institute of Physiology RAS
Email: ivanovapolina19@mail.ru
ORCID iD: 0000-0003-3308-440X
Scopus Author ID: 57203746785
俄罗斯联邦Konstantin Krasnov
Institute of Toxicology of Federal Medico-Biomedical Agency
Email: krasnov_tox@mail.ru
ORCID iD: 0000-0003-1503-2243
AuthorID: 355846
俄罗斯联邦Aleksandr Bespalov
Institute of Toxicology of Federal Medico-Biomedical Agency
Email: lab.mphb@toxicology.ru
ORCID iD: 0000-0003-3537-9930
AuthorID: 726761
俄罗斯联邦参考
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