Bulletin of Nizhnevartovsk State UniversityBulletin of Nizhnevartovsk State University2311-14022686-8784Nizhnevartovsk State University4972010.36906/2311-4444/20-1/08Research ArticleTHE INFLUENCE OF SILICON DIOXIDE «KOVELOS-SORB» ON GROWTH CHARACHTERISTICS OF RHODODENDRON ROSEUM (LOISEL.) REHDER CULTIVATED IN VITRONemtsovaYe. V.-HarinA. V.-RazlugoI. A.-Bryansk State University named after Acad. I.G. Petrovsky150320201485506112020Copyright © 2020, Nemtsova Y.V., Harin A.V., Razlugo I.A.2020The article reveals a stimulating effect of synthetic amorphous silicon dioxide on growth parameters Rhododendron roseum (Loisel.) Rehder, propagating itself in vitro . The purpose of this study was to identify the optimum composition of culture medium on the basis of amorphous silica ‘Kovelos-Sorb’, used for clonal micropropagation of rhododendrons. It was established that it is preferable to add to Anderson culture medium 100 mg/L of amorphous silicon dioxide, which stimulated the growth of mericlone germs Rhododendron roseum (Loisel.) Rehder. In order to stimulate propagation and for receiving a vast amount of propagating material, it was optimal to use Anderson medium which contained 50 mg/L of amorphous silicon dioxide. For establishment of regenerative plants Rhododendron roseum (Loisel.) Rehder in vitro, it was optimal to use Anderson medium which contained synthetic amorphous silicon dioxide in amounts of 50-150 mg/L combined with indoleacetic acid in amounts of 1.5 mg/L.Rhododendron roseum (Loisel.) RehderRhododendron roseum (Loisel.) Rehderamorphous silicon dioxideKovelos-Sorbclonal micropropagationаморфный диоксид кремния «Ковелос-Сорб»клональное микроразмножение[Anderson W. C. Propagation of rhododendrons by tissue culture. 1. Development of a culture medium for multiplication of shoots // Comb Proc Int Plant Propag Soc. 1975.][Avestan S., Naseri L. A., Hassanzade A., Sokri S. M., Barker A. V. Effects of nanosilicon dioxide application on in vitro proliferation of apple rootstock // Journal of Plant Nutrition. 2016. Vol. 39. № 6. P. 850-855. https://doi.org/10.1080/01904167.2015.1061550][Balakhnina T., Borkowska A. Effects of silicon on plant resistance to environmental stresses // International Agrophysics. 2013. Vol. 27. № 2. P. 225-232. https://doi.org/10.2478/v10247-012-0089-4][Epstein E. Silicon: its manifold roles in plants // Annals of applied Biology. 2009. Vol. 155. № 2. P. 155-160. https://doi.org/10.1111/j.1744-7348.2009.00343.x][Jones K. W. Silicon in banana plants: uptake, distribution and interaction with the disease fusarium wilt. 2014. https://doi.org/10.14264/uql.2014.470][Moriguchi T., Kozaki I., Matsuta N., Yamaki S. Plant regeneration from grape callus stored under a combination of low temperature and silicone treatment // Plant cell, tissue and organ culture. 1988. Vol. 15. № 1. P. 67-71. https://doi.org/10.1007/BF00039890][Qing W., Huiying H., Jinwen Z. Effect of exogenous silicon and proline on potato plantlet in vitro under salt stress // China Vegetables. 2005. Vol. 9. P. 16-18.][Radovet-Salinschi D., Cachita-Cosma D. Testing the regenerative capacity of Solanum tuberosum var. Gersa explants after 24 weeks storage in living collection // Analele Universitatii din Oradea, Fascicula: Ecotoxicologie, Zootehnie si Tehnologii de Industrie Alimentara. 2012. Vol. 11. P. 423-430.][Sivanesan I., Park S. W. The role of silicon in plant tissue culture // Frontiers in plant science. 2014. Vol. 5. P. 571. https://doi.org/10.3389/fpls.2014.00571]