SUBPOPULATION HETEROGENEITY OF NK CELLS DURING THE GENETIC MODIFICATION FOR SUBSEQUENT USE IN TARGETED THERAPY

M. A. Streltsova; Стрельцова М. А.; S. M. Deev; Деев С. М.; A. M. Sapozhnikov; Сапожников А. М.; M. V. Grechikhina; Гречихина М. В.; J. D. Vavilova; Вавилова Ю. Д.; R. A. Velichinskii; Величинский Р. А.; N. A. Alekseeva; Алексеева Н. А.; A. I. Palamarchuk; Паламарчук А. И.; M. O. Ustiuzhanina; Устюжанина М. О.; A. A. Boyko; Бойко А. А.; E. I. Kovalenko; Коваленко Е. И.

doi:10.31857/S2686738922700068

SUBPOPULATION HETEROGENEITY OF NK CELLS DURING THE GENETIC MODIFICATION FOR SUBSEQUENT USE IN TARGETED THERAPY

Авторлар: Streltsova M.A.¹, Deev S.M.¹^,2, Sapozhnikov A.M.¹, Grechikhina M.V.¹, Vavilova J.D.¹, Velichinskii R.A.¹, Alekseeva N.A.¹, Palamarchuk A.I.¹, Ustiuzhanina M.O.¹^,3, Boyko A.A.¹, Kovalenko E.I.¹
Мекемелер:
1. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
2. I.M. Sechenov First Moscow State Medical University
3. Skolkovo Institute of Science and Technology
Шығарылым: Том 508, № 1 (2023)
Беттер: 41-44
Бөлім: Articles
URL: https://vestnik.nvsu.ru/2686-7389/article/view/651148
DOI: https://doi.org/10.31857/S2686738922700068
EDN: https://elibrary.ru/MVBLMI
ID: 651148

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Аннотация

Obtaining genetically engineered NK cells is one of the developing areas of immunotherapy. In this work, we analyzed the subset heterogeneity of NK cells subjected to retroviral transduction, taking into account the content of adaptive NK cell precursors. It has been shown that subpopulations of KIR2DL2/DL3⁺, as well as CD57^–KIR2DL2/DL3⁺NKG2C⁺, can be modified with greater efficiency than the corresponding subpopulations that do not carry the KIR2DL2/DL3 and NKG2C markers. After genetic modification, the CD57^–KIR2DL2/DL3⁺NKG2C⁺ cells began to express CD57 de novo, acquiring the adaptive NK cell phenotype.

Негізгі сөздер

NK cells, immunotherapy, modification, transduction, adaptive NK cells, proliferative potential, sensitivity to modification

Әдебиет тізімі

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Streltsova M.A., Erokhina S.A., Kanevskiy L.M., et al. Analysis of NK cell clones obtained using interleukin-2 and gene-modified K562 cells revealed the ability of “senescent” NK cells to lose CD57 expression and start expressing NKG2A // PLoS One. 2018. V. 13. P. e0208469.
Fauriat C., Long E.O., Ljunggren H.-G., et al. Regulation of human NK-cell cytokine and chemokine production by target cell recognition // Blood. 2010. V. 115. P. 2167–2176.
Streltsova M.A., Barsov E. V., Erokhina S.A., et al. Retroviral gene transfer into primary human NK cells activated by IL-2 and K562 feeder cells expressing membrane-bound IL-21 // Journal of Immunological Methods. 2017. V. 450. P. 90–94.
Kobyzeva P.A., Streltsova M.A., Erokhina S.A., et al. CD56dimCD57−NKG2C+ NK cells retaining proliferative potential are possible precursors of CD57+NKG2C+ memory-like NK cells // Journal of Leukocyte Biology. 2020. V. 108. P. 1379–1395.
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Том 522, № 1 (2025)

SUBPOPULATION HETEROGENEITY OF NK CELLS DURING THE GENETIC MODIFICATION FOR SUBSEQUENT USE IN TARGETED THERAPY

Толық мәтін

Аннотация

Негізгі сөздер

Авторлар туралы

M. Streltsova

S. Deev

A. Sapozhnikov

M. Grechikhina

J. Vavilova

R. Velichinskii

N. Alekseeva

A. Palamarchuk

M. Ustiuzhanina

A. Boyko

E. Kovalenko

Әдебиет тізімі

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