The article deals with exposed to strong anthropogenic impact of biotopes spiders species community on the territory of Volgograd (South of the European territory of Russia). The material was collected using Barber’s open soil traps with a fixing solution. Using the Simpson diversity index, the state of biotopes was evaluated, and the comparison of the species composition of spiders using cluster analysis based on the qualitative and quantitative aspects of the Jaccard index was carried out. In the course of the exploring, it was found out that the steppe biotopes of this region are least susceptible to anthropogenic load. In urban environment species are replaced with those that are more resistant to anthropogenic stress, but biological diversity is restored over time. The species composition of the near-aquatic biotopes most susceptible to the influence of the environment microclimatic conditions.