The article analyses the larvae of macrozoobenthos insects response to the quality of the water of Bashkir Cis-Ural small rivers. Such analysis is necessary to identify and predict the level of chemicals and their compounds. The analysis of occurrence (in unit shares) of larvae from such orders as Plecoptera, Ephemeroptera, and Trichoptera was performed in the regions of 6 state gauging station on the rivers Ashkadar, Seleuk, Inzer, Zilair and Big Ik in the period of time between 2005 and 2016. For the chemical aspect of this research the following data from the abovementioned rivers were used: content of compounds such as manganese, nickel and iron, oil products, phenols, ammonium nitrogen, copper, zinc, sulfates, chlorides and nitrite nitrogen. The specific combinatory water pollution index was used as a complex indicator. Chemical quality gradations of the water of small rivers in the studied region were defined with the analysis of variance. It was revealed that the occurrence of larvae of some species from Plecoptera, Ephemeroptera, Trichoptera orders correlate with the chemicals determined in waters of the studied rivers by regulatory bodies. A progression was constructed on the decrease of the macrozoobenthos organisms response for indication and regression models to predict pollutant concentration in the water of Bashkir Cis-Ural small rivers.