It is known that the violation of environmental well-being of the region is reflected in the health status. Human saliva was used to determine the parameters that characterize the physiological state of the body. Previously shown that normal values of most indicators of the biochemical composition of saliva depend essentially on the age and gender characteristics and dynamics of the daily fluctuations. However, the dependence of the biochemical composition of saliva from the region of residence so far has not been widely discussed. In this connection, the aim of this study was to investigate the impact of regional features on the biochemical composition of saliva. The study involved 50 healthy volunteers who lived the last 5 years in the city of Omsk, Kurgan, Republic of Kazakhstan and the Khanty-Mansiysk Autonomous Area - Yugra (Khanty-Mansiysk, Nizhnevartovsk, Surgut, and Langepas). Investigation of pH, calcium ion concentration, phosphorous, chloride, albumin, imidazole compounds and alkaline phosphatase activity conducted in saliva. It is shown that regional differences in the concentrations of the test components are statistically significant. It describes the special features of the metabolic processes and should be considered when determining a normal range, and in the process of adaptation of people, changing the region of residence, in order to avoid occurrence of pathological processes.