Structural-functional evaluation of in vivo 2-weeks oral exposure of silver nanoparticles and silver sulfate on the testicle of mice testicle

Cover Page

Cite item

Full Text

Abstract

During the study of the effect of nanoparticles researches aimed at finding out the consequences of their influence on the structural and functional integrity of germ cells, which ensure genetic continuity must occupy the one of the central places. However, in the assessment of the impact of nanosilver on many organs its effect on the testicles in vivo was not studied. That’s why, the aim of the study was to assess in vivo the effect of nanosilver and silver sulfate on the testicle and to determine the correlation between cytogenetic and cytotoxic parameters. The comparative morphological in vivo evaluation of 2-weeks oral exposure of 4 concentrations (0.1; 5; 50 and 500 mg/l) of silver nanoparticles with size of 14.0 nm, stable arabian gum 1:7 by weight, and of 4 similar concentration of silver sulfate on the testicle of mice. The effect of silver nanoparticles and silver sulfate at the concentration of 500 mg/l is shown to lead to a significant increasing of destructured tubules with undifferentiated and depleted spermatic epithelium, significant decreasing in the number of Leydig cells and decreasing trend in the number of spermatidas, spermatozoons and Sertoli cells, which indicates to the inhibition of spermatogenesis in equal measure for both silver nanoparticles and silver sulfate. The pronounced correlation between the increase in the number of spermatidas with apoptosis and decreasing in the number of tubules with spermatozoon, indicating to the mechanism of gonadotoxic action.

About the authors

Natalia N. Belyaeva

Center for Strategic Planning and Management of Medical and Biological Health Risks

Author for correspondence.
Email: belnatnik@mail.ru

MD, PhD, DSci., Prof., Head of Laboratory of Cytohistology of Center for Strategic Planning and Management of Medical and Biological Health Risks, Moscow, 119121, Russian Federation.

e-mail: belnatnik@mail.ru

Russian Federation

V. S. Zhurkov

Center for Strategic Planning and Management of Medical and Biological Health Risks

Email: noemail@neicon.ru
Russian Federation

L. P. Sycheva

A.I. Burnazyan Federal Medical Biophysical Centre

Email: noemail@neicon.ru
Russian Federation

References

  1. Filatov B.N., Bocharova L.I., Klauchek V.V., Maslennikov A.A., Pocheptsev A.Ya., Tochilkina L.P. Production and use of nanomaterials (toxicological and hygienic problems). Medline.ru. 2015; 16(1): 259–66. (in Russian)
  2. Dyrnev A.D. Toxicological of nanoparticls. Byulleten’ eksperimental’noy biologii i meditsiny. 2008; 145(1): 78–80. (in Russian)
  3. Belyaeva N.N., Gasimova Z.M., Mikhaylova R.I., Savostikova O.N., Alekseeva A.V. Morphofunctional cells sessment of dinamics of silver nanoparticles exposure on the rat liver. Gigiena i sanitariya. 2014; 93(1): 51–4. (in Russian)
  4. Belyaeva N.N., Sycheva L.P., Sinitsyna O.O., Savostikova O.N., Yurchenko V.V. Structural-functional cell assessment of nanoparticles in various organs of warm-blooded animals. In: Nanotoxicology: Achievements. Problems and Prospects. Materials of Scientific Conference [Nanotoksikologiya: Dostizheniya. Problemy i perspektivy. Materialy nauchnoy konferentsii]. Volgograd; 2014: 18–20. (in Russian)
  5. Belyaeva N.N., Amanmuradov A.Kh., Gasimova Z.M. Morphofunctional evaluation of the effects of nanosilver and sulphate of silver in the kidneys of mice. In: Rakhmanin Yu.A., ed. Materials of X International Symposium «Human Ecology and biomedical public safety» [Materialy X Mezhdunarodnogo simpoziuma «Ekologiya cheloveka i mediko-biologicheskaya bezopa4snost’ naseleniya»]. Moscow–Yalta; 2015: 31–6. (in Russian)
  6. Belyaeva N.N., Sycheva L.P. Comparative morphological evaluation of in vivo 2-week exposure to silver nanoparticles and silver sulfate on the liver of mice. Gigiena i sanitariya. 2016; 95(9): 899–902. (in Russian)
  7. Belуaeva N.N. Structural-functional evaluation of cellular effects of nanoparticles and nanoenabled products on the organism of warm-blooded animals. In: Rakhmanin Yu.A., ed. Biomedicine of XXI Century: Achievements and Perspective Directions of Development [Biomeditsina KhKh1 veka: dostizheniya i perspektivnye napravleniya razvitiya]. Moscow: RAEN; 2016: 45–52. (in Russian)
  8. Sung J.Y., Ji J.H., Yoon J.U., Kim D.S., Song M.Y., Jeong J., et al. Lung function changes in Sprague-Dawley rats after prolonged inhalation exposure to silver nanoparticles. Inhal. Toxicol. 2008; 20(6): 567–74.
  9. Sung J.Y., Ji J.H., Park J.D., Yoon J.U., Kim D.S., Jeon K.S., et al. Subchronic inhalation toxicity of silver nanoparticles. Toxicol. Sci. 2008; 108(2): 452–61.
  10. Hyun J.S., Lee B.S., Ryu H.Y. Effects of repeated silver nanoparticles exposure on the Histological structure and mucines of nasal respiratore mucosa in rats. Toxicol. Lett. 2008; 182(1-3): 24–8.
  11. Belyaeva N.N. Approaches to morfоfunctional estimation of toxicity of nanoparticles. In: Production and Application of Nanomaterials in Russia Toxicological, Exposure and Regulatory Issues. International Research Workshop Proceedings. Moscow; 2009: 21.
  12. Kim Y.S., Song N.Y., Park J.D., Song R.S., Ryu H.R., Chug Y.H., et al. Subchronic oral toxicity of silver nanoparticles. Part. Fibre Toxicol. 2010; 7: 20.
  13. Johnston H.J., Hutchison G., Christensen F.M., Peters S., Hankin S., Stone V. A review of the in vivo and in vitro toxicity of silver and gold particulates: Particle attributes and biological mechanisms responsible for the observed toxicity. Crit. Rev. Toxicol. 2010; 40(4): 328–46.
  14. Seltenrich N. Nanosilver: Weighing the risks and benefits. Environ. Health Perspect. 2013; 121(7): A220–5.
  15. Katsnelson B.A., Privalova L.I., Gurvich V.B,. Makeyev O.H., Shur V.V., Beikin Y.B.еt al. Comparative in vivo assessment of some adverse bioeffects of equidimensional gold and silver nanoparticles and the attenuation of attenuation of nanosilver,s effects with a complex of unnocuous bioprotectors. Int. J. Mol. Sci. 2013; 14(2): 2449–83.
  16. Wiwanitkit V., Sereemaspun A., Rojanathanes R. Effect of gold nanoparticles on spermatozoa: the first world report. Fertil. Steril. 2009; 91(1): e7–8.
  17. Moretti E., Terzuoli G., Renieri T., Iacoponi F., Castellini C., Giordano C., et al. In vitro effect of gold and silver nanoparticles on human spermatozoa. Andrologia. 2012; 45(6): 392–6.
  18. Taylor U., Barchanski A., Kues W., Barcikowski S., Rath D. Impact of Metal Nanoparticles on Germ Cell Viability and Functionality. Reprod. Dom. Anim. 2012; 47(Suppl. 4): 359–68.
  19. Tiedemann D., Taylor U., Rehbock C., Jakobi J., Klein S., Kues W.A., et al. Reprotoxicity of gold, silver, and gold-silver alloy nanoparticles on mammalian gametes. Analyst. 2014; 139(5): 931–42.
  20. Pavlyuchenkova S.M. The study of patterns development of male sex Sertoli cells in mice after the different experimental influences: Diss. Moscow; 2015. (in Russian)
  21. Pocheptsev A.Ya., Velikorodnaya Yu.I., Filatov B.N. The influence of gold nanoparticles on proliferative activity of cells of rats. Vestnik Volgogradskogo gosudarstvennogo meditsinskogo universiteta. 2012; (2): 47–50. (in Russian)
  22. Sycheva L.P., Murav’eva L.V., Zhurkov V.S., Mikhaylova R.I., Savostikova A.N., Alekseeva A.V., et al. Study of the mutagenic and cytotoxic effects of nanosilver and silver sulphate in germ cells of mice in vivo. Rossiyskie nanotekhnologii. 2016; 11(3-4): 95–100. (in Russian)
  23. Sertoli Cells. Functions of Sertoli cells. Medicalplanet. Available at: http://medicalplanet.su/diagnostica/417.html (in Russian)

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2024 Belyaeva N.N., Zhurkov V.S., Sycheva L.P.


СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС 77 - 37884 от 02.10.2009.