Impedance sensor based on N-acetyl-L-cysteine for evaluation of antioxidant activity against hydroxyl radicals

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Abstract

Hydroxyl radical is the most aggressive and dangerous product of incomplete reduction of oxygen in the body, therefore its determination and the influence of various antioxidants on the inhibition of OH-radicals is an urgent task. An impedance-metric sensor for the determination of hydroxyl radicals based on a graphite electrode modified with electrochemically deposited gold and self-organizing monolayer of N-acetyl-L-cysteine (ACC) has been proposed. The use of ACC causes high selectivity of the sensor, and the use of non-Faraday impedance as a method of analytical signal registration allows to reach the detection limit of OH-radicals of 0.01 nM with a linear range of detectable concentrations of 0.08-8 nM. The developed sensor has been successfully applied to evaluate the activity of some antioxidants (ascorbic acid, glutathione, coenzyme Q10) in relation to OH-radicals.

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About the authors

A. V. Erkovich

National Research Tomsk Polytechnic University

Author for correspondence.
Email: avg48@tpu.ru
Russian Federation, 634050 Tomsk

E. I. Korotkova

National Research Tomsk Polytechnic University

Email: avg48@tpu.ru
Russian Federation, 634050 Tomsk

E. V. Dorozhko

National Research Tomsk Polytechnic University

Email: avg48@tpu.ru
Russian Federation, 634050 Tomsk

A. N. Solomonenko

National Research Tomsk Polytechnic University

Email: avg48@tpu.ru
Russian Federation, 634050 Tomsk

N. V. Aseeva

National Research Tomsk Polytechnic University

Email: avg48@tpu.ru
Russian Federation, 634050 Tomsk

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2. Fig. 1. (a) Cyclic voltammograms in the first derivative mode in 0.1 M KCl solution containing 1 mM [Ru(NH3)6]3+/2+; (b) impedance spectra in the form of logarithmic amplitude-frequency characteristic obtained by the non-Faradayic impedance method. The inset shows the equivalent circuit (Rs is the solution resistance; CPE is the constant phase element).

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3. Fig. 2. (a) Analytical signal recorded by the non-Faraday impedance method; (b) kinetics of destruction of the ACC monolayer by OH radicals in the time range from 120 to 1200 s (n = 3, P = 0.95).

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4. Fig. 3. Selectivity of the ACC/Au/IGE sensor with respect to active oxygen species and biologically active substances: 1O2 (a), ClO– (b), H2O2 (c), D-glucose (d), L-arginine (e), ascorbic acid (e), glutathione (g), quercetin (h), coenzyme Q10 (i), OH• (k) (n = 3, P = 0.95).

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5. Fig. 4. Evaluation of antioxidant activity against hydroxyl radicals (n = 3, P = 0.95).

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