Improvement of selectivity and sensitivity of tetracycline antibiotics analysis based on ESI-MS method with monoquadrupole mass analyser

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Abstract

Methodological approaches that can be applied to improve the metrological characteristics of a monoquadrupole mass analyser for the study of residual content of small organic substances in aqueous media on the example of tetracycline antibiotics are described. To improve the selectivity of the analysis it is suggested to use optimal parameters of electron optics in the source (voltage at the fragmentor) in order to achieve high yield of ion-products. It is shown that the sensitivity of the analysis can be improved by summing the signals of all formed ion-products.

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About the authors

A. V. Streletskiy

Center for Strategic Planning and Management of Medical and Biological Health Risks of the Federal Medical and Biological Agency

Author for correspondence.
Email: AStreletsky@cspfmba.ru
Russian Federation, Moscow

N. S. Antropova

Center for Strategic Planning and Management of Medical and Biological Health Risks of the Federal Medical and Biological Agency

Email: AStreletsky@cspfmba.ru
Russian Federation, Moscow

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Supplementary files

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2. Fig. 1. Mass spectra of fragmentation of protonated chlortetracycline recorded at different values ​​of collision energy with N2 molecules: (a) – no collision, (b) – collision energy of 15 eV.

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3. Fig. 2. Mass spectra of chlortetracycline recorded at different voltage values ​​on the fragmentor, V: (a) 135, (b) 155, (c) 175.

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4. Fig. 3. Graphs of the dependence of the intensity of product ions of tetracycline antibiotics on the collision energy.

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5. Fig. 4. Graphs of the dependence of the intensity of the product ions of tetracycline antibiotics formed directly in the ion source on the voltage on the fragmentor.

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6. Fig. 5. Mass chromatograms of isolated ions of the analytes oxytetracycline, tetracycline, chlortetracycline, metacycline and doxycycline in distilled water, obtained in the SIM and MRM modes of positive ion registration.

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7. Fig. 6. Evaluation of the matrix effect for two positive ion detection modes: SIM (left) and MRM (right).

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