Luminescent determination of dopamine using a camera

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Abstract

It is shown that the interaction of dopamine with fluorescamine can be used as a basis for the luminescent determination of dopamine using a camera, since the product formed as a result of this interaction is characterized by a luminescence maximum in the visible region (485 nm), and a light-emitting diode emitting light in the near ultraviolet region (395 nm) is sufficient to excite the luminescence. The reaction should be carried out at pH 8–8.5 in a phosphate buffer solution for 5 min; fluorescamine should be added to the reaction mixture last. Some analytical characteristics of the determination using a camera are assessed and compared with the characteristics of a similar determination of dopamine using a professional spectrofluorometer and spectrophotometer. The detection limits of dopamine using a camera, spectrophotometer and spectrofluorometer were 1.8, 1.6 and 0.5 μM, the range of determined contents was 5.4–50 μM, 4.8–100 μM, 1.5–100 μM, respectively. The presence of common inorganic ions, the content of which is 10 times higher than the content of dopamine, does not interfere with the determination. The proposed method for determining dopamine can be used for quality control of drugs.

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About the authors

S. A. Gromova

Lomonosov Moscow State University

Email: masha13_1992@mail.ru
Russian Federation, 1, Leninskie Gory, Moscow, 119991

M. V. Matyash

Lomonosov Moscow State University

Author for correspondence.
Email: masha13_1992@mail.ru
Russian Federation, 1, Leninskie Gory, Moscow, 119991

V. V. Apyari

Lomonosov Moscow State University

Email: masha13_1992@mail.ru
Russian Federation, 1, Leninskie Gory, Moscow, 119991

S. G. Dmitrienko

Lomonosov Moscow State University

Email: masha13_1992@mail.ru
Russian Federation, 1, Leninskie Gory, Moscow, 119991

Yu. A. Zolotov

Lomonosov Moscow State University; Kurnakov Institute of General and Inorganic Chemistry of the Russian Academy of Sciences

Email: masha13_1992@mail.ru
Russian Federation, 1, Leninskie Gory, Moscow, 119991; 31, Leninsky Ave., Moscow, 119991

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2. Scheme 1. Interaction between dopamine and fluorescamine.

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3. Fig. 1. Normalised 3D luminescence spectrum of the product of the reaction of dopamine with fluorescamine. sdophamine = 25 μM, sfluorescamine = 200 μM, pH 8.5, V = 5.0 ml).

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4. Fig. 2. Dependence of luminescence intensity on solution pH. sdophamine = 25 μM, sfluorexamine = 200 μM, 2.0 ml of universal buffer mixture, V = 5.0 ml, lastly (1) fluorescamine, (2) dopamine were injected.

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5. Fig. 3. Effect of (a) nature of buffer solution, (b) interaction time, (c) fluorescamine concentration on the luminescence intensity of the product of reaction of dopamine with fluorescamine. (a), (b); 0.5 ml of buffer solution with pH 8.5; phosphate buffer solution (b), (c); V = 5.0 ml; fluorescamine was introduced last.

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6. Fig. 4. Luminescence (a), (b) luminescence (c) and absorption spectra of dopamine solutions after interaction with fluorescamine. (a), (c) 0-100, (b) 0-10 μM dopamine; (a), (c) 200, (b) 1000 μM fluorescamine; 0.5 ml phosphate buffer solution with pH 8.5; t = 5 min; V = 5.0 ml; fluorescamine was injected last.

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7. Fig. 5. Photographs of graduation (a) solutions of the product of interaction between dopamine and fluorescamine and (b) the graduation relationship for the determination of dopamine obtained with a camera. 0, 2.5, 2.5, 5, 10, 15, 25, 50, 100 µM dopamine; 200 µM fluorescamine; 0.5 ml phosphate buffer solution with pH 8.5; t = 5 min; V = 5.0 ml; fluorescamine was injected last.

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