DOSE-DEPENDENT CHARACTER OF DISTURBANCE OF HEMATOPOIETIC AND IMMUNE SYSTEMS FUNCTION, PRODUCTION OF SOME HORMONES IN EXPERIMENTAL URANIUM ACETATE DIHYDRATE EXPOSURE

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Abstract

The paper presents the results of an experimental study of the dose-dependent nature of functional changes in the body systems under chronic administration of uranyl acetate dihydrate in doses of 0.5 and 5.0 mg/kg per element for 18 weeks. The study was performed on 45 male outbred rats. It has been shown that uranyl acetate dihydrate in a dose of 0.5 mg/kg had no significant effect on hematological parameters. At the same time, activation of bactericidal activity of neutrophils, a decrease in the immunoregulatory index, and an increase in the blood concentration of tumor necrosis factor (TNF-α) have been revealed. The toxicant administered to rats in a dose of 5 mg/kg led to a decrease in the absolute number of erythrocytes, hemoglobin, hematocrit, platelets, the release of myelocytes into the blood, basophilia, monocytosis, the appearance of leukolysis cells and plasmatization of lymphocytes. On the part of the immune system, an increase in the biocidal capacity of neutrophilic granulocytes, TNF-α production, an increase in the number of CD8+ cells, and a reduction in the CD4+/CD8+ ratio have been found. Uranyl acetate dihydrate had a dose-dependent effect only on the number of cytotoxic T-lymphocytes, T-cells with the CD4+CD8+ phenotype, on the immunoregulatory index, and on the level of TNF-α. Hyperglycemia and glucosuria were also dose-dependent. An increase in glucose in the blood and urine indicated a violation of carbohydrate metabolism and kidney function. There was a decrease in the concentration of thyroxine, testosterone and an increase in the level of insulin. Uranyl acetate dihydrate led to the development of insulin resistance. The level of hormones did not depend on the dose of the toxicant administered to the animals.

About the authors

К. I. Stosman

Institute of Toxicology of the Federal Medical and Biological Agency; Smorodintsev Research Institute of Influenza

Author for correspondence.
Email: labtox6@rambler.ru

Stosman Kira Iosifovna

192019, Saint Petersburg

197376, Saint Petersburg

Russian Federation

К V. Sivak

Smorodintsev Research Institute of Influenza

Email: kvsivak@gmail.com

Sivak Konstantin Vladimirovich

197376, Saint Petersburg

Russian Federation

Т. A. Rassokha

Smorodintsev Research Institute of Influenza

Email: tat.rassoxa2012@yandex.ru

Rassokha Tatyana Anatolievna

197376, Saint Petersburg

Russian Federation

Т. N. Savateeva-Lubimova

Smorodintsev Research Institute of Influenza

Email: drugs_safety@mail.ru

Savateeva-Lubimova Tatyana Nikolaevna

197376, Saint Petersburg

Russian Federation

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