SYNTHESIS OF DNA IN PERIPHERAL BLOOD LYMPHOCYTES IN SHEEP AT DIETARY CHRONIC INTAKE OF LEAD

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Abstract

Studies were conducted on 12 sheep of Romanov breed, 1-1.5 years old and weighing 33,0 ± 1.1 kg,. The animals were equally divided at random into 4 groups: group 1st received basic control diet (control group), 2nd, 3rd and 4th groups daily received feed with lead nitrate in concentrations of 5, 25 and 150 mg/kg during the 90 day experiment. With account of daily diet of 2 kg, a daily intake of metal was on average of 10, 50 and 300 mg respectively. A total and reparative DNA synthesis was determined in the peripheral blood lymphocytes. Blood samples were taken from the jugular vein before feeding on the 7th, 14th, 28th, 42nd, 56th, 70th and 90th days of investigation. The chronic lead intake with the diet in sheep was characterized by changes in the total and reparative DNA synthesis in peripheral blood lymphocytes. It was observed that the low level of lead in diet (5 mg/kg) activates or inhibits the synthesis and reparation processes depending on the date of the study. The activation of the total and reparative DNA synthesis in peripheral blood lymphocytes was observed with increase of the metal content in the diet (25 and 50 mg/kg). The most pronounced changes were registered in animals of the 4th group (150 mg/kg). It is supposed that the activation of the reparation process makes the main contribution to the intensity of the total DNA synthesis in peripheral blood lymphocytes at a chronic exposure to lead.

About the authors

Eldeniz Balabek ogly Mirzoev

«All-Russian Institute of Agricultural Radiology and Agroecology»

Author for correspondence.
Email: noemail@neicon.ru

Д.б.н., вед. н. с. лаборатории радиобиологии и экотоксикологии сельскохозяйственных животных ФГБНУ ВНИИРАЭ, 249032, г. Обнинск, Калужской обл., Российская Федерация

e-mail: mirzoev.ed@yandex.ru

Russian Federation

Vladimir Olegovich Kobyalko

«All-Russian Institute of Agricultural Radiology and Agroecology»

Email: noemail@neicon.ru

К.б.н., зав. лабораторией радиобиологии и экотоксикологии сельскохозяйственных животных ФГБНУ ВНИИРАЭ, 249032, г. Обнинск, Калужской обл., Российская Федерация

e-mail: kobyalko@yandex.ru

Russian Federation

Irina Vladimirovna Polyakova

«All-Russian Institute of Agricultural Radiology and Agroecology»

Email: noemail@neicon.ru

Мл.н.с. лаборатории радиобиологии и экотоксикологии сельскохозяйственных животных ФГБНУ ВНИИРАЭ, 249032, г. Обнинск, Калужской обл., Российская Федерация

e-mail: irinaamchenkina@mail.ru

Russian Federation

Olga Aleksandrovna Gubina

«All-Russian Institute of Agricultural Radiology and Agroecology»

Email: noemail@neicon.ru

Н.с. лаборатории радиобиологии и экотоксикологии сельскохозяйственных животных ФГБНУ ВНИИРАЭ, 249032, г. Обнинск, Калужской обл., Российская Федерация

e-mail: Olgubina@yandex.ru

Russian Federation

Natalya Aleksandrovna Frolova

«All-Russian Institute of Agricultural Radiology and Agroecology»

Email: noemail@neicon.ru

К.б.н., н.с. лаборатории радиобиологии и экотоксикологии сельскохозяйственных животных ФГБНУ ВНИИРАЭ, 249032, г. Обнинск, Калужской обл., Российская Федерация

e-mail: nafc@yandex.ru

Russian Federation

References

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Copyright (c) 2015 Mirzoev E.B., Kobyalko V.O., Polyakova I.V., Gubina O.A., Frolova N.A.


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