CYTOME ANALYSIS OF EFFECTS OF GENOMIC INSTABILITY INDUCED BY DIFFERENTLY SIZED SILVER NANOPARTICLES IN HUMAN WHOLE BLOOD CULTURE

Мұқаба

Дәйексөз келтіру

Толық мәтін

Аннотация

A high bactericidal activity of silver nanoparticles (NHS) suggests the possibility of their use in the preparation of drinking water. On the example of human peripheral blood lymphocytes cultured in micronucleus test with cytochalasin B, effects of genome instability were investigated in a concentration range of 0.005-5.0 mg / l of silver nanoparticles (NHS) with a size of 14.3 ± 0.2 nm (Ag14) and 100.0 ± 11.0 nm (AG100), stabilized with gum acacia and for comparison , those of Ag2SO4 (silver ions). In the same range of concentrations . All substances were suspended or dissolved in drinking water.The results showed that the NPS had genotoxic and cytotoxic effects that made them unsuitable to improve the quality of drinking water. Effects of genome instability determined by the frequency of dividing cells with micronuclei and nucleoplasmic bridges, as well as inhibition of mitotic activity, decrease of proliferative activity and increasing duration of the cell cycle came down in series of Ag2SO4 >> Ag100 >> Ag14. However, frequency rise of asymmetric 3-nucleated cells due to aneuploidy induction was the most characteristic of Ag14 particles. 

Авторлар туралы

F. Ingel

A.N. Sysin Research Institute of Human Ecology and Environmental Health, of Ministry of Health of Russia

Хат алмасуға жауапты Автор.
Email: fainaingel@mail.ru
119121, Moscow Ресей

E. Krivtsova

A.N. Sysin Research Institute of Human Ecology and Environmental Health, of Ministry of Health of Russia

Email: e_k_krivcova@mail.ru
119121, Moscow Ресей

N. Yurtseva

A.N. Sysin Research Institute of Human Ecology and Environmental Health, of Ministry of Health of Russia

Email: urtseva@mail.ru
119121, Moscow Ресей

O. Savostikiva

A.N. Sysin Research Institute of Human Ecology and Environmental Health, of Ministry of Health of Russia

Email: savon05@yandex.ru
119121, Moscow Ресей

A. Alekseeva

A.N. Sysin Research Institute of Human Ecology and Environmental Health, of Ministry of Health of Russia

Email: al.anna@list.ru
119121, Moscow Ресей

Әдебиет тізімі

  1. Ahamed m., Alsalhi m.s., siddiqui m.K. Silver nanoparticle applications and human health.//Clin Chim Acta. 2010, v.411, № 23-24:1841-1848
  2. De Jong W.h., borm p.J.A. Drug delivery and nanoparticles: application and hazards // Int J of Nanomedicine, 2008, v.3, № 2:133-149
  3. Mosin O. V., Ignatov And. Methods of receiving fine nanoparticles of colloidal silver the Internet magazine “NAUKOVEDENIYE” Release 3, May – June 2014 http:// naukovedenie.ru 85TVN314
  4. Xu L , Li X , Takemura T , Hanagata N , Wu G M S V, Chou LL Genotoxicity and molecular response of silver nanoparticle (NP)-based hydrogel. //J Nanobiotechnology. 2012, v.10, № 1:16-18;
  5. De Lima R, Seabra AB, Durán N. Silver nanoparticles: a brief review of cytotoxicity and genotoxicity of chemically and biogenically synthesized nanoparticles. J Appl Toxicol. 2012, Jun 13. doi: 10.1002/jat.2780;
  6. Lansdown A.B.G. A Pharmacological and Toxicological Profile of Silver as an Antimicrobial Agent inMedical Devices. Review Article //Advances in Pharmacological Sciences, Volume 2010, Article ID 910686, doi: 10.1155/2010/910686
  7. Katsnelson B. A., Privalova L.I., Sutunkova M.P., Gurvich V.B., Minigaliyeva I.A., Loginova N. V., Kireev E.P., Schur W. Ja., Shishkina E.V., Beykin Ya.B., Pichugova S.V., Makeev O. G., Valamina I.E. The main results of toxicological experiments “in vivo” with some metal - oxidic nanoparticles. Toxicological vestnik, 2015 No. 3, S.26-40.
  8. Katsnelson b.A, Privalova L.I., Sutunkova M.P., Gurvich V.B., Loginova N.V., Minigalieva I.A., Kireyeva E.P., Shur V.Y., Shishkina E.V., Beikin Y.B., Makeyev O.H., Valamina I.E. Some inferences from in vivo experiments with metal and metal oxide nanoparticles: the pulmonary phagocytosis response, subchronic systemic toxicity and genotoxicity, regulatory proposals, searching for bioprotectors (a self-overview). Int J Nanomedicine. 2015 Apr 16; 10:3013-29. doi: 10.2147/IJN.S80843. eCollection 2015.
  9. Tomankova K., Horakova J., Harvanova M., Malina l., Soukupova J., Hradilova S., Kejlova Kristina, Malohlava Libor Licman Jakub, Dvorakova Marketa, Jirova Dagmar, Kolarova H. Cytotoxicity, cell uptake and microscopic analysis of titanium dioxide and silver nanoparticles in vitro, Food and Chemical Toxicology (2015), doi: 10.1016/j.fct.2015.03.027
  10. Thiago Verano-Braga, Rona Miethling-Graff, Katarzyna Wojdyla, Adelina RogowskaWrzesinska,Jonathan R. Brewer, Helmut Erdmann, and Frank Kjeldsen. Insights into the Cellular Response Triggered by Silver Nanoparticles Using Quantitative Proteomics. ACS NANO,• February 2014, www.acsnano.org. doi: 10.1021/nn4050744
  11. Hay RT (2005). “SUMO: a history of modification”. Mol. Cell 18 (1): 1–12. doi: 10.1016/j.molcel.2005.03.012
  12. Asharani p. v., Kah Mun Grace Low ‡, Hande M. P., Valiyaveettil S. Cytotoxicity and Genotoxicity of Silver Nanoparticles in Human Cells //ACS Nano, 2009, 3 (2), pp 279–290
  13. Xiu ZM, Zhang QB, Puppala HL, Colvin VL, Alvarez PJ. Negligible particle-specific antibacterial activity of silver nanoparticles. Nano Lett. 2012; 12 (8):4271-5. doi: 10.1021/nl301934w.
  14. Kittler, S. C. G.; Gebauer, J. S.; Diendorf, J.; Treuel, L.; Ruiz, L.; Gonzalez-Calbet, J. M.; ValletRegi, M.; Zellner, R.; Köller, M. Epple, M. The Influence of Proteins on the Dispersability and Cell-Biological Activity of Silver Nanoparticles. J. Mater Chem. 2009, 20:512–518
  15. Chong-xin Huang, Bo Lv, and Yue Wang. Protein Phosphatase 2A Mediates Oxidative Stress Induced Apoptosis in Osteoblasts Mediators Inflamm. 2015; 2015: 804260.10.1155/2015/804260
  16. Lim Hui Kheng, Asharani P. V., Prakash Hande M. Enhanced genotoxicity of silver nanoparticles in DNA repair deficient mammalian cells Frontiers in Genetics, 2012, v. 3, p.104. doi: 10.3389/fgene.2012.00104
  17. Vecchio G, Fenech M, Pompa PP, Voelcker NH. Lab-on-a-chip-based high-throughput screening of the genotoxicity of engineered nanomaterials. Small. 2014; 10 (13):2721- 34. doi: 10.1002/smll.201303359.
  18. Spivak I.M. Action of the alkilating agents and ionizing radiation on human cells with hereditary violations of DNA reparation. Abstract of the thesis for degree of Candidate of Biology, St. Petersburg, 1998, S.26.
  19. McLuckie KI, Crookston RJ, Gaskell M, Farmer PB, Routledge MN, Martin EA, Brown K. Mutation spectra induced by alphaacetoxytamoxifen-DNA adducts in human DNA repair proficient and deficient (xeroderma pigmentosum complementation group A) cells//Biochemistry. 2005 v.44, № 22, P.8198-8205; 19.
  20. Kroes R, Galli C, Munro I, Schilter B, Tran L, Walker R, Würtzen G.Threshold of toxicological concern for chemical substances present in the diet: a practical tool for assessing the need for toxicity testing. Food Chem Toxicol. 2000, v.38, № 2-3:255-312
  21. Gonzalez l., Sanderson B.J. S. and Kirsch-Volders M. Adaptations of the in vitro MN assay for the genotoxicity assessment of nanomaterials [Journal] / Mutagenesis - 2011.-1. Vol. 26:185-191.
  22. Ingel F.I., Guskov A.S., Yurchenko V. V., Krivtsov E.K., Yurtseva N. A. Indicators of proliferative activity and their correletions with genetic damages of lymphocytes of human blood cultivated in cytokinetic block//the Bulletin of the Russian Academy of Medical Science, 2006, No. 4:41-46.
  23. Ingel F.I. Prospects of use of micronuclear test on human blood lymphocytes cultivated using cytokinetic block. Part 1. Proliferation of cages. Ecological genetics 2006,v. IV, No. 3: 7-19
  24. Fenech M. Cytokinesis-block micronucleus assay evolves into a “cytome” assay of chromosomal instability, mitotic dysfunction and cell death //MutRes. - 2006.-Vol.537, №7:127-132.
  25. Fenech M., Chang W.P., Kirsch-Volders M. et al. HUMN project: detailed description of the scoring criteria for the cytokinesis-block micronucleus assay using isolated human lymphocyte cultures //Mut Res, 2003. Vol. 534, P.65-75.
  26. Gonzalez l., Sanderson B.J. S. and Kirsch-Volders M. Adaptations of the in vitro MN assay for the genotoxicity assessment of nanomaterials [Journal] / Mutagenesis. - 2011. - 1: Vol. 26:185-191.
  27. Statement on genotoxicity assessment of nanomaterials* and experimental considerations by committee on mutagenicity of chemicals in food, consumer products and the environment (com) http://www.iacom.org.uk/statements/documents/nanomaterialsfinal2012_000.pdf
  28. OECD guideline for the testing of chemicals. Proposal for updating Test Guideline 487. In Vitro Mammalian Cell Micronucleus Test. http://www.oecd.org/chemicalsafety/testing/50108793.pdf
  29. Moshkov N. E., Ingel F. I., Krivtsov G. G. A morphometry of cellular nuclei for screening of the effects of genomic instability in micronuclear test on human blood lymphocytes cultivated in cytokinetic block// Nanotechnology and health protection, 2012, v. IV, No. 1 (10):24-32
  30. Ingel f., Zatsepina O., Stekhin A., Yakovleva G., Savostikova O., Alekseeva A., Iksаnova T. Electrochemically activated water induced effects of genomic instability in various living objects in vitro and in vivo. Central European Journal of Occupational and Environmental Medicine 2013, Vol. 1, Iss.7, 1:143 http://esciencecentral.org/journals/electrochemicallyactivated-tap-water-induced-effects-ofgenomic-instability-in-various-living-objectsin-vitro-and-in-vivo-2329-6879.1000143.php?aid=23292 http://dx.doi.org/10.4172/2329-6879.1000143
  31. Koshelev K.K., Kosheleva O.K., Pautov V.P., Gorovenko V. I., Svistunov M. G. Preparations of the KND-M colloidal metals for medicine. Nanotechnology and health protection, 2010, No. 2, S.36-38
  32. Smith LE, Nagar S, Kim GJ, Morgan WF. Radiation-induced genomic instability: radiation quality and dose response. Health Phys. 2003, Jul; 85 (1):23-29.
  33. Titenko-Holland N., Jacob R. A., Shang N., Balaraman A., Smith M. T. Micronuclei in lymphocytes and exfoliated buccal cells of postmenopausal women with dietary changes in folate. Mutat. Res., 1998. v. 417, N 2 – 3:101 – 114.
  34. Timoshevsky VA., Nazarenko S. A. Biologic indication of mutagenic influences and human genetic instability by the accounting of numerical chromosomal violations. Vestnic VOGIS, 2006, T.10, No. 3, S.530-540.
  35. Wise JP Sr, Goodale BC, Wise SS, Craig GA, Pongan AF, Walter RB, Thompson WD, Ng AK, Aboueissa AM, Mitani H, Spalding MJ, Mason MD. Silver nanospheres are cytotoxic and genotoxic to fish cells. Aquat Toxicol. 2010, v.97, № 1:34-41.Healthcare of Russia, 119121, Moscow, Russian Federation

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© Ingel F.I., Krivtsova E.K., Yurtseva N.A., Savostikiva O.N., Alekseeva A.V., 2017


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