INVESTIGATION OF THE LEVEL OF GENE EXPRESSION OF SUBUNITS OF GABA RECEPTOR AFTER ADMINISTRATION OF KLOFLUBICIN

The article presents the results of determining the level of expression of genes encoding GABA receptor subunits GABRA1, GABRB2, and GABRG2 in the hippocampus of rats 24 hours after a single intramuscular administration of the antagonist of the chloro-ion channel of GABA receptor of kloflubicin in a dose of LD₂₅, LD₄₀, LD₅₀, and LD₇₅. It is revealed that kloflubicin has no influence on the relative level of GABRG2 gene expression. At the same time, the relative level of GABRA1 gene expression increases 7,5, 7,0, and 5,0 times after administration of kloflubicin in a dose of LD₄₀, LD₅₀, and LD₇₅, respectively. The relative level of GABRB2 gene expression also increases 3,6 and 2,6 times after administration of kloflubicin in a dose of LD₅₀ and LD75, respectively. It is assumed that increase in the level of gene expression of GABRA1 and GABRB2 in rats after administration of kloflubicin in doses above LD₂₅ is a compensatory reaction of the body to the effect of physiologically active substance, and subunits encoded by these genes α1 and β2, respectively, are included in the mechanism of convulsive effect.