Comparative analysis of cytology of rat bronchoalveolar lavage fluid after single exposure to metal oxide nanoparticles

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Аннотация

Introduction. Human production activities (metallurgical, mining, electronics production and processing, batteries) are related to air pollution of the working area and the environments of complex composition aerosols. Among the aerosol components, ultrathin particles of the nanometer range (including metal nano-oxides) are considered to be the most dangerous. Due to their prevalence, study to assess the cytotoxicity of metal oxide nanoparticles are relevant.

Material and methods. CuO, PbO, CdO, Fe2O3, NiO nanoparticle (NP) suspension were obtained by laser ablation. The study was done on white outbred female rats. A single intratracheal instillation of different chemical NPs was performed (in dose 0.5 mg/animal); control animals received a similar amount of deionized water. A day after the NP instillation bronchoalveolar lavage (BAL) was carried out with the subsequent assessment of its cytological indices.

Results. The cytotoxic action of the studied NPs, based on the cytological indices of the BAL fluid, is changed as follows (from greater to lesser): CuO NP > CdO NP > PbO NP > NiO NP > Fe2O3 NP.

Limitations. Such physical characteristics of nanoparticles as solubility in water and biological fluids, charge, adsorption capacity, resistance to aggregation, hydrophobicity, adhesion to surfaces, and the ability to generate free radicals have not been studied. Extrapolation of data from rodents to humans shall be done with caution, since cytotoxicity has been characterized only based on the main cellular parameters.

Conclusion. Bronchoalveolar lavage cytology can be used as an effective screening method for the cytotoxic effect of NPs.

Compliance with ethical standards. The animal study protocols were approved by the Institutional Ethics Committee of the Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers (Protocol No. 2 of April 20, 2020).

Contribution of the authors:
Sutunkova M.P., Minigalieva I.A., Privalova L.I. — the concept and design of the study, editing;
Klinova S.V., Ryabova Yu.V. — conducting a study, the collection and processing of the material, writing text, editing;
Tazhigulova A.V. — conducting a study, the collection and processing of the material, editing.
All co-authors — approval of the final version of the article, responsibility for the integrity of all parts of the article.

Acknowledgment. Authors express their gratitude to the team of the Ural Center for Collective Use “Modern Nanotechnologies” of the Ural Federal University named after the first President of Russia B.N. Yeltsin and personally to the director of the Ural Center for Collective Use “Modern Nanotechnologies”, professor, doctor of physical and mathematical sciences Vladimir Yakovlevich Shur, for the synthesis of suspensions, studied nanoparticles of a given characteristic on the basis of the “Modern Nanotechnologies” center.

Conflict of interests. The authors declare no conflict of interests.

Funding. The study had no sponsorship.

Date of receipt: May 18, 2023 / Date of acceptance for printing: December 3, 2023 / Date of publication: December 29, 2023

 

Авторлар туралы

Svetlana Klinova

The Yekaterinburg Medical Research Center for Prophylaxis and Health Protection of Industrial Workers

Хат алмасуға жауапты Автор.
Email: klinova.svetlana@gmail.com
ORCID iD: 0000-0002-0927-4062

Researcher, Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers of Rospotrebnadzor, Yekaterinburg, Russian Federation

e-mail: klinova.svetlana@gmail.com

Ресей

Marina Sutunkova

The Yekaterinburg Medical Research Center for Prophylaxis and Health Protection of Industrial Workers

Email: sutunkova@ymrc.ru
ORCID iD: 0000-0002-1743-7642

MD, director of The Yekaterinburg Medical Research Center for Prophylaxis and Health Protection of Industrial Workers of Rospotrebnadzor, 620014, Yekaterinburg, Russian Federation

e-mail: sutunkova@ymrc.ru

Ресей

Ilzira Minigalieva

The Yekaterinburg Medical Research Center for Prophylaxis and Health Protection of Industrial Workers

Email: ilzira-ilzira@ymrc.ru
ORCID iD: 0000-0002-0097-7845

Doctor of Biological Sciences, Head of the Department of Toxicology and Bioprevention of The Yekaterinburg Medical Research Center for Prophylaxis and Health Protection of Industrial Workers of Rospotrebnadzor, 620014, Yekaterinburg, Russian Federation

e-mail: ilzira-ilzira@ymrc.ru  

Ресей

Yulia Ryabova

The Yekaterinburg Medical Research Center for Prophylaxis and Health Protection of Industrial Workers

Email: ryabova@ymrc.ru
ORCID iD: 0000-0003-2677-0479

Head of the Laboratory of Scientific Fundamentals of Bioprevention of The Yekaterinburg Medical Research Center for Prophylaxis and Health Protection of Industrial Workers of Rospotrebnadzor, 620014, Yekaterinburg, Russian Federation

e-mail: ryabova@ymrc.ru

Ресей

Anastasiya Tazhigulova

The Yekaterinburg Medical Research Center for Prophylaxis and Health Protection of Industrial Workers

Email: tazhigulovaav@ymrc.ru
ORCID iD: 0000-0001-9384-8550

Junior Researcher, Laboratory of Scientific Fundamentals of Bioprevention of The Yekaterinburg Medical Research Center for Prophylaxis and Health Protection of Industrial Workers of Rospotrebnadzor, 620014, Yekaterinburg, Russian Federation

e-mail: tazhigulovaav@ymrc.ru  

Ресей

Larisa Privalova

The Yekaterinburg Medical Research Center for Prophylaxis and Health Protection of Industrial Workers

Email: privalovali@yahoo.com
ORCID iD: 0000-0002-1442-6737

MD, professor, Chief Researcher of Department of Toxicology and Bioprevention of The Yekaterinburg Medical Research Center for Prophylaxis and Health Protection of Industrial Workers of Rospotrebnadzor, 620014, Yekaterinburg, Russian Federation

e-mail: privalovali@yahoo.com 

Ресей

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© Klinova S.V., Sutunkova M.P., Minigalieva I.A., Ryabova Y.V., Tazhigulova A.V., Privalova L.I., 2024


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