Use of bovine spermatozoa as a rapid test for mitochondrial toxicity of T-2 toxin and deltamethrin

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Introduction. Modern requirements for the introduction of drugs into clinical practice include a test for mitochondrial toxicity. Mitochondria are targets of many pharmaceutical and therapeutic agents that can damage them and lead to changes in morphology and function. Spermatozoa have one of the largest ratios of mitochondria to body size, they lack the cytoplasm between the mitochondria and the plasma membrane, so they are a good potential model for a rapid test for mitochondrial toxicity.

Material and methods. The aim of our work was to study the possibility of using Bos taurus taurus spermatozoa to assess mitochondrial toxicity. T-2 toxin and deltamethrin were used as toxins. The main parameters studied were the level of mitochondrial potential (using the MitoTracker dye), sperm motility and their relationship.

Results. We found that there is a strong correlation between the motility of bovine spermatozoa and the mitochondrial potential of their mitochondria (R>0.87, p<0.05). The mitochondrial toxicity of deltamethrin has been confirmed, although to a much lesser extent than that of the T-2 toxin. In addition, certain patterns were found in the distribution of active zones of the mitochondrial potential in bull spermatozoa.

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Leisan Timerbulatova

Volga Region State Academy of Physical Culture, Sport and Tourism

编辑信件的主要联系方式.
Email: L_Z_93@mail.ru
ORCID iD: 0000-0001-6321-6130

Junior researcher of the research institute

俄罗斯联邦

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СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС 77 - 81728 от 11 декабря 2013.