The content of nitrogen, phosphorus in cereals and dicot grasses leaves and features of their rhizosphere microflora in multi-age fly ash dumps

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The nitrogen (N) and phosphorus (P) content was studied in the leaves of 6 cereals and 18 non-legume dicotyledonous grass species, as well as directly in the substrates of non-recultivated areas of two different age fly ash dumps. The total number of microorganisms and nitrogen fixers capable of phosphate solubilization and indole-3-acetic acid (IAA) synthesis was analyzed in the rhizosphere of two pioneer (Alopecurus aequalis, Ranunculus sceleratus) and two late successional (Poa pratensis, R. auricomus) plant species. Higher N and P content and lower N/P ratio were found in the leaves of dicot grasses compared to cereals. The number of nitrogen fixers in the rhizosphere of dicots was higher, which coincided with the higher content of total nitrogen in it. The proportion of nitrogen fixers with an increased ability to phosphate solubilize and IAA produce was significantly higher in the early stages of ash dump overgrowth.

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A. Betekhtina

Ural Federal University named after the first President of Russia B. N. Yeltsin

编辑信件的主要联系方式.
Email: a.a.betekhtina@urfu.ru
俄罗斯联邦, Mira St., 19, Yekaterinburg, 620002

O. Nekrasova

Ural Federal University named after the first President of Russia B. N. Yeltsin

Email: a.a.betekhtina@urfu.ru
俄罗斯联邦, Mira St., 19, Yekaterinburg, 620002

A. Malakheeva

Ural Federal University named after the first President of Russia B. N. Yeltsin

Email: a.a.betekhtina@urfu.ru
俄罗斯联邦, Mira St., 19, Yekaterinburg, 620002

O. Voropaeva

Ural Federal University named after the first President of Russia B. N. Yeltsin

Email: a.a.betekhtina@urfu.ru
俄罗斯联邦, Mira St., 19, Yekaterinburg, 620002

M. Maleva

Ural Federal University named after the first President of Russia B. N. Yeltsin

Email: a.a.betekhtina@urfu.ru
俄罗斯联邦, Mira St., 19, Yekaterinburg, 620002

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2. Fig. 1. Nitrogen content in leaves of monocotyledonous and dicotyledonous plant species on ash dumps of different ages: (a) – young ash dump, (b) – old ash dump. Point – arithmetic mean (n = 26), shaded area – SE, end marks – SD. Different Latin letters indicate significant differences between variants at p < 0.05.

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3. Fig. 2. Phosphorus content in leaves of monocotyledonous (cereals) and dicotyledonous herbaceous plants in ash dumps of different ages at VTGRES: (a) – young ash dump, (b) – old ash dump. Point – arithmetic mean value (n = 26), shaded area – SE, end marks – SD. Different Latin letters indicate reliable differences between variants at p < 0.05.

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4. Fig. 3. Percentage distribution of nitrogen-fixing rhizobacteria strains isolated from monocots (inner volume circle) and dicots (outer circle with dots) plants with different phosphate solubilization abilities in young (a) and old (b) ash dumps.

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5. Fig. 4. Percentage distribution of strains of nitrogen-fixing rhizobacteria isolated from monocots (inner volume circle) and dicots (outer circle with dots) plants with different ability to synthesize IAA in young (a) and old (b) ash dumps.

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