DIFFERENT PROTEIN GROUPS INVOLVED IN TRANSCRIPTION REGULATION IN GENES OF DEVELOPMENT AND HOUSEKEEPING IN  DROSOPHILA

I. F. Zhimulev; Жимулев И. Ф.; T. Yu. Vatolina; Ватолина Т. Ю.; G. V. Pokholkova; Похолкова Г. В.; O. V. Antonenko; Антоненко О. В.; M. V. Maltseva; Мальцева М. В.

doi:10.31857/S2686738923700348

DIFFERENT PROTEIN GROUPS INVOLVED IN TRANSCRIPTION REGULATION IN GENES OF DEVELOPMENT AND HOUSEKEEPING IN DROSOPHILA

Authors: Zhimulev I.F.¹, Vatolina T.Y.¹, Pokholkova G.V.¹, Antonenko O.V.¹, Maltseva M.V.¹
Affiliations:
1. Institute of Molecular and Cellular Biology, Siberian Branch of the Russian Academy of Sciences
Issue: Vol 512, No 1 (2023)
Pages: 438-443
Section: Articles
URL: https://vestnik.nvsu.ru/2686-7389/article/view/651069
DOI: https://doi.org/10.31857/S2686738923700348
EDN: https://elibrary.ru/SRXVUU
ID: 651069

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Abstract

Antibodies to histone modifications and an insulator protein involved in the processes of transcription initiation and elongation are mapped in Drosophila polytene chromosomes. The CHRIZ protein (chromatin insulator) and H3K36me3 histone modification (RNA elongation) are detected only in the localization of housekeeping genes (interbands and gray bands of polytene chromosomes) and never in the regions of development genes (black bands and large puffs arising from them). Antibodies to H3S10P histone modification, which is associated with the initial elongation of the RNA strand during transcription, are found exclusively in small puffs, but not in housekeeping gene localization sites or large ecdysone-induced puffs, where housekeeping genes are localized. Antibodies to H4R3me2 histone modification (a co-repressor of the ecdysone receptor) are detected only in large α-induced puffs.

Keywords

histone modifications H3K36, H3S10P, H4R3me2, CHRIZ protein, housekeeping genes, developmental genes, gene promoters, polytene chromosomes, Drosophila

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