THE ROLE OF 20-HYDROXYECDYSONE IN THE CONTROL OF CARBOHYDRATE LEVELS IN  DROSOPHILA MELANOGASTER  UNDER SHORT-TERM HEAT STRESS

M. A. Bobrovskikh; Бобровских М. А.; N. E. Gruntenko; Грунтенко Н. Е.

doi:10.31857/S2686738923600206

THE ROLE OF 20-HYDROXYECDYSONE IN THE CONTROL OF CARBOHYDRATE LEVELS IN DROSOPHILA MELANOGASTER UNDER SHORT-TERM HEAT STRESS

Authors: Bobrovskikh M.A.¹, Gruntenko N.E.¹
Affiliations:
1. Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences
Issue: Vol 511, No 1 (2023)
Pages: 387-390
Section: Articles
URL: https://vestnik.nvsu.ru/2686-7389/article/view/651093
DOI: https://doi.org/10.31857/S2686738923600206
EDN: https://elibrary.ru/JDUCWJ
ID: 651093

Cite item

Full Text

Open Access
Restricted Access

Access granted
Restricted Access

Subscription Access

Abstract
About the authors
References
Supplementary files
Statistics

Abstract

It is known that 20-hydroxyecdysone is one of the most important hormonal regulators of development, reproduction and adaptation to unfavourable conditions in insects. Here, we show for the first time that exogenous 20-hydroxyecdysone increases the content of two main insect carbohydrates, trehalose and glucose, in Drosophila melanogaster females both in normal conditions and under short-term heat stress. It has been found that the levels of both trehalose and glucose increase after thirty minutes of heat exposure, and return to their original levels after an hour and a half. A scheme of hormonal regulation of carbohydrate content under heat stress, involving 20-hydroxyecdysone, juvenile hormone and dopamine, is suggested.

Keywords

Drosophila melanogaster, 20-hydroxyecdysone, carbohydrate metabolism, trehalose, glucose, heat stress

About the authors

M. A. Bobrovskikh

Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences

Email: nataly@bionet.nsc.ru
Russian Federation, Novosibirsk

N. E. Gruntenko

Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences

Author for correspondence.
Email: nataly@bionet.nsc.ru
Russian Federation, Novosibirsk

References

Раушенбах И.Ю. Нейроэндокринная регуляция развития насекомых в условиях стресса: Генетико-физиологические аспекты. // Новосибирск, 1990. Rauschenbach I.Yu. Neyroendokrinnaya regulyatsiya razvitiya nasekomykh v usloviyakh stressa: Genetikofiziologicheskie aspekty[Neuroendocrine Regulation of Insect Development under Stress:Genetic and Physiological Aspects]. // Novosibirsk: Nauka Publ., 1990. (in Russian)
Gruntenko N.E., Rauschenbach I.Y. Interplay of JH, 20E and biogenic amines under normal and stress conditions and its effects on reproduction // J. Insect Physiol. 2008. V. 54. № 6. P. 902–908. https://doi.org/10.1016/j.jinsphys.2008.04.004
Miyashita A., Adamo S.A. Stayin’ Alive: Endocrinological stress responses in insects. In: Advances in Invertebrate Endocrinology. Toronto, 2020.
Broughton S.J., Piper M.D.W., Ikeya T., et al. Longer lifespan, altered metabolism, and stress resistance in Drosophila from ablation of cells making insulin-like ligands // PNAS. 2005. V. 102. № 8. P. 3105–3110.
Gruntenko N.E., Rauschenbach I.Y. The role of insulin signalling in the endocrine stress response in Drosophila melanogaster // Gen. Comp. Endocrinol. 2018. V. 258. P. 134–139.
Rulifson E.J., Kim S.K., Nusse R. Ablation of insulinproducing neurons in flies: growth and diabetic phenotypes // Science. 2002. V. 296. № 5570. P. 1118–1120.
Karpova E.K., Eremina M.A., Pirozhkova D.S., et al. Stress-related hormones affect carbohydrate metabolism in Drosophila females // Arch. Insect Biochem. Physiol. 2019. V. 101. № 1. e21540.
Keshan B., Thounaojam B., Kh S.D. Insulin and 20-hydroxyecdysone action in Bombyx mori: Glycogen content and expression pattern of insulin and ecdysone receptors in fat body // Gen. Comp. Endocrinol. 2017. V. 241. P. 108–117.
Zhang S.Y., Gao H., Askar A., et al. Steroid hormone 20-hydroxyecdysone disturbs fat body lipid metabolism and negatively regulates gluconeogenesis in Hyphantria cunea larvae // J. Insect Sci. 2022.
Wang X.P., Huang Z., Li Y-L. et al. Krüppel-like factor 15 integrated autophagy and gluconeogenesis to maintain glucose homeostasis under 20-hydroxyecdysone regulation // PLoS Genet. 2022. V. 18. № 6. e1010229.
Li Y.N., Lio Y-B., Xie X-Q., et al. The modulation of trehalose metabolism by 20-hydroxyecdysone in Antheraea pernyi (Lepidoptera: Saturniidae) during its diapause termination and post-termination period // J. Insect. Sci. 2020. V. 20. № 5.
Yu J., Wang H., Chen W., et al. 20-Hydroxyecdysone and receptor interplay in the regulation of hemolymph glucose level in honeybee (Apis mellifera) larvae // Metabolites. 2023. V. 13. № 1.
Hou Y., Wang X-L., Saha T.T., et al. Temporal coordination of carbohydrate metabolism during mosquito reproduction // PLoS Genet. 2015. V. 11. № 7. e1005309.
Gruntenko N.E., Laukhina O.V., Rauschenbach I.Yu., et al. Role of D1- and D2-like receptors in age-specific regulation of juvenile hormone and 20-hydroxyecdysone levels by dopamine in Drosophila // J. Insect. Physiol. 2012. V. 58. № 12. P.1534–1540.
Musselman L.P., Fink J.L., Narzinski K., et al. A high-sugar diet produces obesity and insulin resistance in wild-type Drosophila // Dis. Models. Mech. 2011. V. 4. № 6. P. 842–849.