Study Using the Micronuclear Test of Radiosensitivity and Induction Radiation of Adaptive Response in Peripheral Blood Lymphocytes of Patients with Oncological Diseases

Cover Page

Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

Radiosensitivity to low and medium doses of X-ray radiation (XR) and the ability to induce a radiation adaptive response (RAR) of lymphocytes during in vitro irradiation of peripheral blood of patients with cancer were studied. The criterion for cytogenetic damage was the frequency of micronuclei (MN) in cytochalasin-blocked binucleate lymphocytes in culture. It was found that the spontaneous level of cytogenetic damage in the lymphocytes of patients was 2.6 times higher than in healthy volunteers, and there was also significant interindividual variability in values compared to the control cohort. There were no differences in mean values for radiosensitivity to low and medium doses of XR between the study groups. There was no correlation between the spontaneous level of MN in lymphocytes and the radiosensitivity of individuals in both groups. RAR was induced with the same frequency and to the same extent in lymphocytes from both patients and healthy individuals.

About the authors

V. E. Balakin

Physical-Technical Center of Lebedev Physical Institute, Russian Academy of Sciences

Email: strelnikova.ns@lebedev.ru

Corresponding Member of the RAS

Russian Federation, Protvino

O. M. Rozanova

Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences

Email: strelnikova.ns@lebedev.ru
Russian Federation, Pushchino

N. S. Strelnikova

Physical-Technical Center of Lebedev Physical Institute, Russian Academy of Sciences

Author for correspondence.
Email: strelnikova.ns@lebedev.ru
Russian Federation, Protvino

E. N. Smirnova

Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences

Email: strelnikova.ns@lebedev.ru
Russian Federation, Pushchino

T. A. Belyakova

Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences; Institute for High Energy Physics named by A.A. Logunov of National Research Centre “Kurchatov Institute”

Email: strelnikova.ns@lebedev.ru
Russian Federation, Pushchino; Protvino

References

  1. Пелевина И.И., Петушкова В.В., Бирюков В.А., и др. Роль “немишенных эффектов” в реакции клеток человека на радиационное воздействие. // Радиационная биология. Радиоэкология. 2019. Т. 59. №3. С. 261–273.
  2. Когарко И.Н., Аклеев А.В., Петушкова В.В., и др. Адаптивный ответ как критерий оценки индивидуальной радиочувствительности человека. Обзор. // Радиация и риск. 2022. Т. 31. №1. С. 93–104.
  3. Rödel F., Frey B., Gaipl U., et al. Modulation of inflammatory immune reactions by low-dose ionizing radiation: molecular mechanisms and clinical application. // Curr Med Chem. 2012. V. 19. №12. Р. 1741–1750
  4. Balakin V.E., Zaichkina S.I., Rozanova O.M., et al., Low-dose ionizing radiation inhibits the aging-related rise in accumulation of spontaneous cytogenetic abnormalities: the genome-stabilizing effect. // Dokl Biol Sci. 2000. V. 374. P. 488-490.
  5. Ghiassi-Nejad M., Mortazavi S.M., Cameron J.R., et al. Very high background radiation areas of Ramsar, Iran: preliminary biological studies. // Health physics. 2002. V. 82. №1. P. 87–93.
  6. Петушкова В.В., Пелевина И.И., Серебряный А.М., и др. Некоторые подходы к анализу структуры адаптивного ответа при профессиональном облучении. // Радиация и риск. 2020. Т. 29. № 4. С. 97–105.
  7. Fenech M., Chang W.P., Kirsch-Volders M., et al. HUMN project: detailed description of the scoring criteria for the cytokinesis-block micronucleus assay using isolated human lymphocyte cultures. // Mutat Res. 2003. V. 534. №1-2. P. 65–75.
  8. World Medical Association. “World Medical Association Declaration of Helsinki: ethical principles for medical research involving human subjects”. // JAMA. 2013. V. 310 №20. P. 2191–2194.
  9. Zaichkina S.I., Rozanova O.M., Aptikaeva G.F., et al. Low doses of gamma-radiation induce nonlinear dose responses in Mammalian and plant cells. // Nonlinearity Biol Toxicol Med. 2004. V. 2. №3. P. 213–221.
  10. Zaichkina S.I., Aptikaeva G.F., Rozanova O.M., et al. Action of chronic irradiation on the cytogenetic damage of human lymphocyte culture. // Environ Health Perspect. 1997. V.105. P. 1441–1443.
  11. Fenech M. The in vitro micronucleus technique. // Mutat Res. 2000. V. 455. №1-2. P. 81–95.
  12. Andreassi M.G., Barale R., Iozzo P., et al. The association of micronucleus frequency with obesity, diabetes and cardiovascular disease. // Mutagenesis. 2011. V. 26. №1. P. 77–83.
  13. Bonassi S., Znaor A., Ceppi M., et al. An increased micronucleus frequency in peripheral blood lymphocytes predicts the risk of cancer in humans. // Carcinogenesis. 2007. V. 28. №3. P. 625–631.
  14. Bonassi S., El-Zein R., Bolognesi C., et al. Micronuclei frequency in peripheral blood lymphocytes and cancer risk: evidence from human studies. // Mutagenesis. 2011. V. 26. №1. P. 93–100.
  15. Rozanova O.M., Smirnova E.N., Belyakova T.A., et al. A Study on the Radiosensitivity and Induction of Adaptive Response in Peripheral Blood Lymphocytes of Patients with Secondary Immunodeficiency Syndrome. // Biophysics. 2023. V. 68. №5. P. 767–773.
  16. Gantenberg H.W., Wuttke K., Streffer C., et al. Micronuclei in human lymphocytes irradiated in vitro or in vivo. // Radiat Res. 1991. V. 128. №3. P. 276–281.
  17. Пелевина И.И., Алещенко А.А., Антощина М.М., и др. Индивидуальная вариабельность в проявлении адаптивного ответа клеток человека на воздействие ионизирующей радиации. Подходы к ее определению. // Радиационная биология, Радиоэкология. 2007. Т. 47. №6. С. 658–666.
  18. Ivanova T.I., Khorokhorina V.A., Sychenkova N.I., et al. Proceedings of 3rd Russian Conference with International Participation “Radiobiological Foundations of Radiation Therapy”. 2019. P. 66–68.
  19. Cortese F., Klokov D., Osipov A., et al. Vive la radiorésistance!: converging research in radiobiology and biogerontology to enhance human radioresistance for deep space exploration and colonization. // Oncotarget. 2018. V. 9. №18. P. 14692–14722.
  20. Barquinero J.F., Barrios L., Caballín M.R., et al. Occupational exposure to radiation induces an adaptive response in human lymphocytes. // Int J Radiat Biol. 1995. V. 67. №2. P. 187–191.
  21. Gerić M., Popić J., Gajski G., et al. Cytogenetic status of interventional radiology unit workers occupationally exposed to low-dose ionising radiation: A pilot study. // Mutat Res Genet Toxicol Environ Mutagen. 2019. V. 843. P. 46–51.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2024 Russian Academy of Sciences