Search and verification of  Betula nana L.  hybrid samples in the Urals

S. O. Medvedeva; Медведева С. О.; O. E. Cherepanova; Черепанова О. Е.; I. V. Petrova; Петрова И. В.; A. Yu. Teptina; Тептина А. Ю.

doi:10.31857/S2686738925020047

Search and verification of Betula nana L. hybrid samples in the Urals

Authors: Medvedeva S.O.¹, Cherepanova O.E.¹, Petrova I.V.¹, Teptina A.Y.²
Affiliations:
1. Botanical Garden, UrB RAS
2. Ural Federal University
Issue: Vol 521, No 1 (2025)
Pages: 193-197
Section: Articles
URL: https://vestnik.nvsu.ru/2686-7389/article/view/684004
DOI: https://doi.org/10.31857/S2686738925020047
ID: 684004

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Abstract

Using the flow cytometry method, hybrid samples were verified in B. nana populations, most of which were identified in the northern part of the Ural Mountains. Analysis of the ITS1 region nucleotide sequences obtained using the Sanger method showed low differentiation of B. nana and B. pubescens species associated with current and ancestral hybridization processes. Analysis of intragenomic polymorphism of ITS1 sequences using Illumina technology revealed the presence of rDNA of both B. nana and B. pubescens in first-generation interspecific hybrids genome.

Keywords

rDNA, ITS1, dwarf birch, downy birch, NGS, flow cytometry

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References

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