Targeted therapy in combination with an agent that enhances terminal differentiation of cancer cells

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Abstract

Currently, for the treatment of oncological diseases, in addition to standard treatment methods, an approach is being developed in which terminal differentiation of cancer cells is induced with the help of pharmacological drugs, leading to an increase in the therapeutic effect under the influence of traditional treatment methods. A number of examples of the use of dimethyl sulfoxide (DMSO) as a safe adjuvant that stimulates the differentiation of various types of the cancer cells, have been described in the literature. In this paper, we propose a method for elimination of human ovarian cancer cell adenocarcinomas SKOV3.ip1, including targeted therapy based on the HER2-specific toxin DARP-LoPE and DMSO as an enhancing agent for terminal differentiation of cancer cells.

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About the authors

Е. I. Shramova

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russian Academy of science; P.N. Lebedev Physical Institute of the Russian Academy of Sciences

Author for correspondence.
Email: shramova.e.i@gmail.com
Russian Federation, Moscow; Moscow

G. M. Proshkina

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russian Academy of science

Email: shramova.e.i@gmail.com
Russian Federation, Moscow

I. N. Zavestovskaya

P.N. Lebedev Physical Institute of the Russian Academy of Sciences; National Research Center “Kurchatov Institute”

Email: shramova.e.i@gmail.com
Russian Federation, Moscow; Moscow

S. M. Deyev

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russian Academy of science; P.N. Lebedev Physical Institute of the Russian Academy of Sciences; National Research Center “Kurchatov Institute”; Sechenov First Moscow State Medical University (Sechenov University)

Email: shramova.e.i@gmail.com

Academician of the RAS

Russian Federation, Moscow; Moscow; Moscow; Moscow

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2. Fig. 1. Combined effect of the differentiating agent DMSO and the targeted HER2-specific toxin DARP-LoPE on 2D culture of SKOV3.ip1 cells (A) and 3D spheroids of mixed type consisting of SKOV3.ip1 cells, endothelial-derived EA.hy926 cells and BJ-5TA stromal fibroblasts (B).

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