Physiological effect of 24-epibrassinolide and its conjugate with succinic acid on the resistance of rapeseed plants to chloride salinity

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Abstract

It has been shown for the first time that exogenous 24-epibrassinolide (EBL) and a conjugate synthesized on its basis, which is 2,3,22,23-tetraester of EBL and succinic acid, at a concentration of 10 nM almost equally mitigate the negative effects of salt stress (150 mM NaCl) on rapeseed plants, maintaining photosynthetic activity and growth parameters. Specificity of the action of the studied compounds in regulating the resistance of rapeseed plants to NaCl is noted. EBL against the background of chloride salinity increases the content of carotenoids, superoxide dismutase and peroxidase activity, while conjugate EBL increases only peroxidase activity. Pretreatment of plants for 4 hours before stress was more effective than the introduction of the studied regulators simultaneously with the stressor.

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About the authors

L. V. Kolomeichuk

National research Tomsk State University

Author for correspondence.
Email: kolomeychuklv@mail.ru
Russian Federation, Tomsk

R. P. Litvinovskaya

Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus

Email: kolomeychuklv@mail.ru
Belarus, Minsk

V. А. Khripach

Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus

Email: kolomeychuklv@mail.ru
Belarus, Minsk

V. V. Kuznetsov

National research Tomsk State University; Timiryazev Institute of Plant Physiology, Russian Academy of Sciences

Email: kolomeychuklv@mail.ru

Corresponding Member of the RAS

Russian Federation, Tomsk; Moscow

М. V. Efimova

National research Tomsk State University

Email: kolomeychuklv@mail.ru
Russian Federation, Tomsk

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2. Fig. 1. General structure of 24-epibrassinolide and its conjugate.

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3. Fig. 2. Effect of 24-epibrassinolide (EBL, 10 nM) and its conjugate (TGS EBL, 10 nM) against the background of chloride salinity (NaCl, 150 mM) on growth parameters of rapeseed plants. *p < 0.05 when compared with the control.

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4. Fig. 3. Effect of 24-epibrassinolide (10 nM, EBL) and its conjugate (TGS EBL, 10 nM) under chloride salinity (150 mM, NaCl) on the fluorescence parameters of photosystem II. *p < 0.05 when compared with the control. A – maximum photochemical efficiency; B – effective quantum yield; C – quantum yield of unregulated dissipation of light energy.

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5. Fig. 4. Effect of 24-epibrassinolide (EBL, 10 nM) and its conjugate (TGS EBL, 10 nM) under chloride salinity (NaCl, 150 mM) on the content of photosynthetic pigments in the leaves of rapeseed plants. *p < 0.05 when compared with the control.

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6. Fig. 5. Effect of 24-epibrassinolide (EBL, 10 nM) and its conjugate (TGS EBL, 10 nM) under chloride salinity (NaCl, 150 mM) on the level of lipid peroxidation in leaves, stems and roots of rapeseed plants. *p < 0.05 when compared with the control.

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7. Fig. 6. Effect of 24-epibrassinolide (EBL, 10 nM) and its conjugate (TGS EBL, 10 nM) under chloride salinity (NaCl, 150 mM) on the proline content in leaves, stems and roots of rapeseed plants. *p < 0.05 when compared with the control.

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