Regenerative potential of platelet-rich plasma in diabetes mellitus

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Abstract

The study explores in vitro the regenerative potential of platelet-rich plasma (PRP) from patients with type 1 diabetes mellitus: in a series of experiments they add PRP to the culture medium of dermal fibroblasts and evaluate in dynamics the metabolic (including the production of reactive oxygen species), migration and proliferation activity of the cells, and they also determine the growth factors and exosomes levels in PRP and the medium. They reveal a decrease in the proliferative, prooxidant and toxic effects of PRP from patients with diabetes mellitus. They are characterized with a decrease in metabolic activity and cell viability in culture with an increase in the percentage of necrosis, while the migration properties of the cells were not impaired. The presence of diabetes mellitus in a patient is also accompanied by a decrease in the stimulating effect of his PRP on dermal fibroblasts in the secretion of growth factors. These effects were most significant in elderly people with diabetes.

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About the authors

T. I. Vlasova

Federal State Budgetary Educational Institution of Higher Education “National Research Ogarev Mordovia State University”

Author for correspondence.
Email: v.t.i@bk.ru
Russian Federation, Moscow

E. P. Brodovskaya

Federal State Budgetary Educational Institution of Higher Education “National Research Ogarev Mordovia State University”

Email: v.t.i@bk.ru
Russian Federation, Moscow

К. S. Madonov

Federal State Budgetary Educational Institution of Higher Education “National Research Ogarev Mordovia State University”

Email: v.t.i@bk.ru
Russian Federation, Moscow

V. P. Ageev

Federal State Budgetary Educational Institution of Higher Education “National Research Ogarev Mordovia State University”

Email: v.t.i@bk.ru
Russian Federation, Moscow

A. P. Abelova

Federal State Budgetary Educational Institution of Higher Education “National Research Ogarev Mordovia State University”

Email: v.t.i@bk.ru
Russian Federation, Moscow

S. I. Pinyaev

Federal State Budgetary Educational Institution of Higher Education “National Research Ogarev Mordovia State University”

Email: v.t.i@bk.ru
Russian Federation, Moscow

А. P. Vlasov

Federal State Budgetary Educational Institution of Higher Education “National Research Ogarev Mordovia State University”

Email: v.t.i@bk.ru
Russian Federation, Moscow

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Supplementary files

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2. Fig. 1. Scheme of the experiment for assessing the effects of PRP from patients with type 1 diabetes of different age groups on the culture of human dermal fibroblasts (ROS – reactive oxygen species, ELISA – enzyme-linked immunosorbent assay, CS – culture medium; PRP from patients of different groups was assessed according to a single protocol).

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3. Fig. 2. The viability index (according to the MTT test) of the culture at different observation periods after adding PRP from donors of different groups (* – reliability of differences between the DM1 group and the K1 group at p < 0.001, # – reliability of differences between the DM2 group and the K2 group at p < 0.001).

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4. Fig. 3. Morphological picture and mechanisms of cell death (percentage distribution).

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5. Fig. 4. Fluorescence microscopy data of hTERT-HDFa cells, 24 h after PRP addition. Scale bar = 100 μm. AO and PI staining: AO stains the nuclei of living cells green, apototic cells – bright green and yellow; PI penetrates only into cells with damaged membrane integrity (necrosis marker) and stains the nucleus red.

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6. Fig. 5. Percentage of defect closure (A) and defect structure (B), 24 hours after PRP application. Scale bar = 100 µm.

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7. Fig. 6. Exosome fractions (CD9/CD81) in the PRP sample of subjects from different groups (K1, K2, CD1, CD2) (% of 10,000 registered events).

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Note

Presented by Academician of the RAS I.V. Reshetov


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