Intravenous administration of heterodimeric phospholipase A2 HDP-1 disturbs cardiovascular system functions and biochemical composition of blood in anesthesiated rats

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Heterodimeric phospholipase A2 HDP-1 from the venom of Nikolsky viper consists of two non-covalently bound subunits – HDP-1P and HDP-1In, the first of which exhibits lipolytic activity, while the second does not. The subunits have close molecular masses and similar amino acid sequences. Administration of HDP-1 and HDP-1P to anesthetized rats is accompanied by prolonged hypotensive effects, the intensity of which depends on the dose. With the administration of HDP-1P, the effect develops more slowly than in the case of HDP-1 containing both subunits, while individual HDP-1In does not affect hemodynamic parameters. It was found using electrocardiography that HDP-1 and HDP-1P affect the functioning of the rat heart, causing arrhythmia, which may indicate their ability to affect the innervation of the heart. The administration of HDP-1 and its subunits also leads to changes in biochemical blood parameters, indicating their damaging effect on cells and internal organs.

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作者简介

A. Ismailova

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

Email: utkin@ibch.ru

Branch

俄罗斯联邦, Moscow; Pushcino

M. Severyukhina

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences; the Federal State Budgetary Educational Institution of Higher Education “Russian Biotechnological University (ROSBIOTECH)”

Email: utkin@ibch.ru

Branch, Pushchino branch

俄罗斯联邦, Moscow; Pushchino; Puschino

E. Shaykhutdinova

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

Email: utkin@ibch.ru

Branch

俄罗斯联邦, Moscow; Pushcino

N. Perepechenova

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

Email: utkin@ibch.ru

Branch

俄罗斯联邦, Pushchino

V. Starkov

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

Email: utkin@ibch.ru
俄罗斯联邦, Moscow

I. Dyachenko

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences; the Federal State Budgetary Educational Institution of Higher Education “Russian Biotechnological University (ROSBIOTECH)”

Email: utkin@ibch.ru

Branch, Pushchino branch

俄罗斯联邦, Moscow; Pushchino; Pushchino

V. Tsetlin

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

Email: utkin@ibch.ru

Corresponding Member of the RAS

俄罗斯联邦, Moscow

Yu. Utkin

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

编辑信件的主要联系方式.
Email: utkin@ibch.ru
俄罗斯联邦, Moscow

参考

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2. Fig. 1. Changes in hemodynamic parameters after administration of HDP-1 (A, D), HDP-1P (B, E), and HDP-1In (C, E). * p ≤ 0.05 by Wilcoxon test for HDP-1 group at a dose of 250 μg/kg compared with baseline values ​​of the group; # p ≤ 0.05 by Wilcoxon test for HDP-1 groups at a dose of 400 μg/kg compared with baseline values ​​of the group; & p ≤ 0.05 by Wilcoxon test for HDP-1P groups at a dose of 250 μg/kg compared with baseline values ​​of the group.

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3. Fig. 2. Effect of HDP-1 and its subunits on the ECG of anesthetized rats. (A) standard ECG of the control group; (B) a single extrasystole upon administration of HDP-1 at a dose of 250 μg/kg; (C) cardiac arrest and bradycardia upon administration of HDP-1P at a dose of 250 μg/kg; (D) cardiac arrest manifested by an increase in the intervals between contractions upon administration of HDP-1P at a dose of 250 μg/kg.

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