New cellular partners of HIV-1 integrase and their role in viral replication

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Cellular proteins, partners of viral enzymes, are involved in the replication of the human immunodeficiency virus type I (HIV-1) at various stages. Thus, the viral enzyme integrase, participating in several stages of the viral cycle, interacts with various cellular proteins. A number of them are already known, and for some the mechanism of action has been established, but the search for integrase cellular partners continues. In this work, the identification of cellular partners of HIV-1 integrase has been carried out by the cross-linking method followed by mass spectrometry (XL-MS). Twelve new potential integrase partners have been identified, and some of them have been examined for their effect on the early stages of HIV-1 replication.

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Y. Agapkina

Lomonosov Moscow State University; Lomonosov Moscow State University

编辑信件的主要联系方式.
Email: agapkina@belozersky.msu.ru

Department of Chemistry, A.N. Belozersky Research Institute of Physico-Chemical Biology

俄罗斯联邦, Moscow; Moscow

T. Ponomareva

Lomonosov Moscow State University

Email: agapkina@belozersky.msu.ru

A.N. Belozersky Research Institute of Physico-Chemical Biology

俄罗斯联邦, Moscow

M. Vdovina

Lomonosov Moscow State University

Email: agapkina@belozersky.msu.ru

Department of Chemistry

俄罗斯联邦, Moscow

R. Ziganshin

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry

Email: agapkina@belozersky.msu.ru
俄罗斯联邦, Moscow

A. Rozina

Lomonosov Moscow State University

Email: agapkina@belozersky.msu.ru

A.N. Belozersky Research Institute of Physico-Chemical Biology

俄罗斯联邦, Moscow

A. Anisenko

Lomonosov Moscow State University; Lomonosov Moscow State University

Email: agapkina@belozersky.msu.ru

Department of Chemistry, A.N. Belozersky Research Institute of Physico-Chemical Biology

俄罗斯联邦, Moscow; Moscow

M. Gottikh

Lomonosov Moscow State University; Lomonosov Moscow State University

Email: agapkina@belozersky.msu.ru

Department of Chemistry, A.N. Belozersky Research Institute of Physico-Chemical Biology

俄罗斯联邦, Moscow; Moscow

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2. Fig. 1. Effect of changing the intracellular concentration of DnaJA1, Cofilin 1, 14-3-3ξ/δ, and CacyBP proteins on the efficiency of cell transduction with HIV-1-based lentiviral vector. A. Western blot analysis results for protein overexpression. Visualization with antibodies to 3xFLAG and actin. B. Relative mRNA level of proteins after knockdown with siRNA. C. Relative luminescence level for protein overexpression. D. Relative luminescence level for protein knockdown. p-values ​​were calculated using the Mann–Whitney U test. * p-value <0.05, ** p-value <0.01, *** p-value <0.001, **** p-value <0.0001.

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Presented by Academician of the RAS O.A. Dontsova


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