INFLUENCE OF THE CULTIVATION CONDITIONS OF THE GLIOBLASTOMA NEUROSPHERE ON THE EXPRESSION OF MALAT1 AND LINCROR LONG NON-CODING RNA GENES

D. V. Mazur; Мазур Д. В.; A. V. Mishanova; Мишанова А. В.; T. F. Kovalenko; Коваленко Т. Ф.; M. I. Shakhparonov; Шахпаронов М. И.; N. V. Antipova; Антипова Н. В.

doi:10.31857/S2686738922600960

INFLUENCE OF THE CULTIVATION CONDITIONS OF THE GLIOBLASTOMA NEUROSPHERE ON THE EXPRESSION OF MALAT1 AND LINCROR LONG NON-CODING RNA GENES

Authors: Mazur D.V.¹, Mishanova A.V.¹, Kovalenko T.F.¹, Shakhparonov M.I.¹, Antipova N.V.¹^,2
Affiliations:
1. Institute of Bioorganic Chemistry named after M.M. Shemyakin and Yu.A. Ovchinnikov
2. Department of Biology and Biotechnology, Higher School of Economics
Issue: Vol 510, No 1 (2023)
Pages: 243-246
Section: Articles
URL: https://vestnik.nvsu.ru/2686-7389/article/view/651103
DOI: https://doi.org/10.31857/S2686738922600960
EDN: https://elibrary.ru/QFAHLS
ID: 651103

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Abstract

ABSTRACT Glioblastoma multiforme (GBM) is the most aggressive malignant brain tumor. One of the reasons for the resistance of MGB to treatment is the extreme heterogeneity of the tumor and, in particular, the presence of cancer stem cells (CSCs) in the population of glioblastoma cells. In this work, we investigated the effect of conditions that reduce the proportion of CSCs in the GBM cell population on the levels of long noncoding RNAs (lincROR and MALAT1) involved in the formation of the phenotype of glioblastoma cancer stem cells. We have shown that culturing under conditions that cause a decrease in cell stemness (when fetal calf serum is added to the culture medium) affected the content of these transcripts: in the cells of most of the analyzed lines, a decrease in the level of the positive stemness regulator lincROR and an increase in the content of MALAT1 were noted.

Keywords

glioblastoma, cancer stem cells, lincROR, MALAT1

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