Contact inhibition of proliferation is accompanied by expression of the PHF10D subunit of the chromatin remodeling complex PBAF in mouse and human cell lines

Cover Page

Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription Access

Abstract

PHF10 is a subunit of the PBAF complex, which regulates the expression of many genes in developing and maturing organisms. PHF10 has four isoforms that differ in domain structure. The PHF10A isoform, containing a DPF domain at the C-terminus and 46 amino acids at the N-terminus, is necessary for the expression of gene proliferation; the functions of the other isoforms are less studied. In this work, we have established that upon contact inhibition of mouse and human cell proliferation caused by the establishment of a tight junction and adherence junction between cells, the expression of the PHF10A isoform stops and instead the PHF10D isoform is expressed, which does not contain DPF-domain and N-terminal sequence. The function of the PHF10D isoform may be associated with the establishment of intercellular contacts.

Full Text

Restricted Access

About the authors

Yu. P. Simonov

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences

Email: so2615nat@gmail.com

Department of Transcription Factors

Russian Federation, Moscow

V. V. Tatarskiy

Institute of Gene Biology, Russian Academy of Sciences

Email: so2615nat@gmail.com
Russian Federation, Moscow

S. G. Georgieva

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences

Email: so2615nat@gmail.com

academician of the RAS, Department of Transcription Factors

Russian Federation, Moscow

N. V. Soshnikova

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences; Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences

Author for correspondence.
Email: so2615nat@gmail.com

Department of Transcription Factors

Russian Federation, Moscow; Moscow

References

  1. Chen K., Yuan J., Sia Y., Chen Z. Mechanism of action of the SWI/SNF family complexes // Nucleus. 2023. V. 14.
  2. Local A., Huang H., Albuquerque C. P., Singh N., Lee A. Y., Wang W., Wang C., Hsia J. E., Shiau A. K., Ge K., Corbett K. D., Wang D., Zhou H., Ren B. Identification of H3K4me1-associated proteins at mammalian enhancers // Nat. Genet. 2018. V. 50. P. 73–82.
  3. Chugunov A. O., Potapova N. A., Klimenko N. S., Tatarskiy V. V., Georgieva S. G., Soshnikova N. V. Conserved Structure and Evolution of DPF Domain of PHF10-The Specific Subunit of PBAF Chromatin Remodeling Complex // Int. J. Mol. Sci. 2021. V. 22.
  4. Brechalov A. V., Georgieva S. G., Soshnikova N. V. Mammalian cells contain two functionally distinct PBAF complexes incorporating different isoforms of PHF10 signature subunit // Cell Cycle. 2014. V. 13. P. 1970–1979.
  5. Lessard J., Wu J. I., Ranish J. A., Wan M., Winslow M. M., Staahl B. T., Wu H., Aebersold R., Graef I. A., Crabtree G. R. An essential switch in subunit composition of a chromatin remodeling complex during neural development // Neuron. 2007. V. 55. P. 201–215.
  6. Viryasova G. M., Tatarskiy Jr V. V., Sheynov A. A., Tatarskiy E. V., Sud’ina G.F., Georgieva S. G., Soshnikova N. V. PBAF lacking PHD domains maintains transcription in human neutrophils // Biochim. Biophys. Acta Mol. Cell Res. 2019. V. 1866. P. 118525.
  7. Soshnikova N. V., Azieva A. M., Klimenko N., Khamidullina A. I., Feoktistov A. V., Sheynov A. A., Brechalov A. V., Tatarskiy V. V., Georgieva S. G. A novel chromatin-remodeling complex variant, dcPBAF, is involved in maintaining transcription in differentiated neurons // Front. Cell Dev. Biol. 2023. V. 11. P. 1271598.
  8. Eagle H., Levine E. M. Growth regulatory effects of cellular interaction // Nature. 1967. V. 213. P. 1102–1106.
  9. McClatchey A.I., Yap A. S. Contact inhibition (of proliferation) redux // Curr. Opin. Cell Biol. 2012. V. 24. P. 685–694.
  10. Fan Y., Meyer T. Molecular control of cell density-mediated exit to quiescence // Cell Rep. 2021. V. 36. P. 109436.
  11. Benaud C. M., Dickson R. B. Adhesion-regulated G1 cell cycle arrest in epithelial cells requires the downregulation of c-Myc // Oncogene. 2001. V. 20. P. 4554–4567.
  12. Gumbiner B. M., Kim N.-G. The Hippo-YAP signaling pathway and contact inhibition of growth // J. Cell Sci. 2014. V. 127. P. 709–717.
  13. Aragona M., Panciera T., Manfrin A., Giulitti S., Michielin F., Elvassore N., Dupont S., Piccolo S. A mechanical checkpoint controls multicellular growth through YAP/TAZ regulation by actin-processing factors // Cell. 2013. V. 154. P. 1047–1059.
  14. Zeng Q., Hong W. The emerging role of the hippo pathway in cell contact inhibition, organ size control, and cancer development in mammals // Cancer Cell. 2008. V. 13. P. 188–192.
  15. Banga S. S., Peng L., Dasgupta T., Palejwala V., Ozer H. L. PHF10 is required for cell proliferation in normal and SV40-immortalized human fibroblast cells // Cytogenet. Genome Res. 2009. V. 126. P. 227–242.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download
2. Fig. 1. (a) Schematic representation of the PBAF complex. Different subunits are indicated in different colors and the modules of the complex are indicated (adapted from [1]). (b) The domain organization of the PHF10 isoforms. Various domains are indicated. Green, blue and orange indicate the sequences that distinguish the isoforms.

Download (448KB)
Indexing metadata
3. Fig. 2. (a) Mouse embryonic fibroblasts of MEF 1, 3, 4 and 5 days after passage on a 60 mm Petri dish. (b) Western blotting isoforms of Phf10 (indicated on the right in the upper panel), subunits of the PBAF complex: Baf180, Brg1, Baf155 in MEF lysates selected in different densities. Staining with antibodies to b- tubulin was used as a control of application. A change in the expression of Cyclin D1 (CyclinD1) and P27 characterizes the ability of cells to proliferate. (c) Changes in the mRNA level of Phf10 isoforms containing the DPF domain (Phf10P = Phf10A + Phf10B) and containing a PDSM motif (Phf10-S = Phf10C + Phf10D) at the C-terminus. The Y-axis shows the level change RNA is relative to control when normalized to the expression level of the RPLP0 gene. In all experiments, the measurements were carried out in 3 repetitions and presented as an average ± standard deviation . * – p < 0.005 comparison with control (single-factor analysis of variance with the criterion of multiple comparisons of Dunnet).

Download (539KB)
Indexing metadata
4. Fig. 3. Western blotting of PHF10 isoforms (shown on the right in the upper panel) in lysates of RKO cells selected in different densities. Staining with antibodies to b-tubulin was used as a control of application. Changes in the expression of MYC and P27 characterize the ability of cells to proliferate.

Download (206KB)
Indexing metadata

Copyright (c) 2024 Russian Academy of Sciences