Ontogeny of the epileptic system in rats of the Krushinsky– Molodkina line with genetically determined audiogenic epilepsy

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The development of convulsive proneness of audiogenic epilepsy (AE) and phenotype manifestations of epileptic activity in rats of the Krushinsky – Molodkina (KM) strain, proceeds in parallel with the appearance of seizure EEG patterns followed in this investigation from the age of 2 months age up to 7 months (both in КМ rats). The latencies of the seizure onset reduced with age while the intensity of convulsive seizures increased. In background EEG (no exposure to sound) of KM rats two types of epileptiform discharges (ED) were identified. The first type had the form of high-amplitude generalized “packs” of waves, with animal shuddering. The second type of ED had the form of a generalized non-seizure absence-like “spike-wave” discharges with animal freezing. The duration of single absence-like discharges increased with age. The parallel age-related changes were found between the increase in severity of AE seizure and the increase of generalized absence-like discharges numbers in the forebrain EEG of KM rats, which evidence the development of epileptic system in this strain.

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作者简介

S. Litvinova

Federal State Budgetary Scientific Institution “Federal Research Center for Innovator and Emerging Biomedical and Pharmaceutical Technologies”

编辑信件的主要联系方式.
Email: litvinova_sa@academpharm.ru
俄罗斯联邦, Moscow

A. Yakovleva

Federal State Budgetary Scientific Institution “Federal Research Center for Innovator and Emerging Biomedical and Pharmaceutical Technologies”

Email: litvinova_sa@academpharm.ru
俄罗斯联邦, Moscow

T. Voronina

Federal State Budgetary Scientific Institution “Federal Research Center for Innovator and Emerging Biomedical and Pharmaceutical Technologies”

Email: litvinova_sa@academpharm.ru
俄罗斯联邦, Moscow

N. Gladysheva

Federal State Budgetary Scientific Institution “Federal Research Center for Innovator and Emerging Biomedical and Pharmaceutical Technologies”

Email: litvinova_sa@academpharm.ru
俄罗斯联邦, Moscow

V. Radontseva

Federal State Budgetary Scientific Institution “Federal Research Center for Innovator and Emerging Biomedical and Pharmaceutical Technologies”

Email: litvinova_sa@academpharm.ru
俄罗斯联邦, Moscow

N. Surina

Moscow State University M.V. Lomonosova

Email: litvinova_sa@academpharm.ru

Faculty of Biology

俄罗斯联邦, Moscow

I. Poletaeva

Moscow State University M.V. Lomonosova

Email: litvinova_sa@academpharm.ru

Faculty of Biology

俄罗斯联邦, Moscow

I. Fedotova

Moscow State University M.V. Lomonosova

Email: litvinova_sa@academpharm.ru

Faculty of Biology

俄罗斯联邦, Moscow

A. Durnev

Federal State Budgetary Scientific Institution “Federal Research Center for Innovator and Emerging Biomedical and Pharmaceutical Technologies”

Email: litvinova_sa@academpharm.ru

Academician of the RAS

俄罗斯联邦, Moscow

参考

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2. Fig. 1. Intensity of AE seizures in different periods of ontogenesis of KM rats. LP – latent period of seizure onset. * – p ≤ 0.05 difference from 2 months of KM rats (Kruskal-Wallis test with transition to multiple comparisons according to Dunn).

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3. Fig. 2. ER of two types in KM rats of different ages during 2 hours of EEG recording. Gray columns – polyspikes, shaded columns – absence-like discharges. #, * – p ≤ 0.1, p ≤ 0.05, respectively, the difference from 2 months of KM rats (Kruskal-Ullis criterion with the transition to multiple comparisons according to Dunn).

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4. Fig. 3. Convulsive discharges of the “polyspike” type in the EEG of KM rats. A – 3 months, B – 4 months and C – 7 months animals. Leads: 1 – sensorimotor cortex, left hemisphere, 2 – sensorimotor cortex, right hemisphere, 3 – hypothalamus, 4 – hippocampus. Amplitude calibration – 40 μV/mm.

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5. Fig. 4. Absence-type convulsive discharges in the background EEG of KM rats. Designations as in Fig. 3.

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6. Fig. 5. EEG power spectrum in the background and during absence-like discharge in 2.5–3-month-old rats (A), 4-month-old (B), and 7-month-old (C). The abscissa axis shows the wave frequency from 0.5 to 30 Hz with a step of 1 Hz. L. cortex – sensorimotor cortex, left hemisphere, R. cortex – sensorimotor cortex, right hemisphere, HPT – hypothalamus, HPC – hippocampus.

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