The process the organism individual development implies its adaptation, the patterns and principles of which, among other things, are reflected in the implementation of the male gametophyte. The forest stand natural renewal primarily depends on the indicators of the seed formation success, determined among other things by the pollen characteristics. For species threatened with extinction the problem of renewal stability is especially acute. The paper is aimed to determine the biometric characteristics and the viability level of J. deltoides’s pollen grains in the Mountainous Crimea. In that regard, the following tasks were set: to determine the morphometric parameters of J. deltoides pollen grains, to assess their potential and real viability, and to identify the degree of environmental factors influence on the development of the male reproductive system of J. deltoides in the Mountainous Crimea. Within each trial area, 10 model trees were identified to collect their pollen grains during the flight period. The diameter of pollen grains in two mutually perpendicular planes was measured on temporary acetocarmine preparations. Pollen germination was carried out using the hanging drop method. A new methodology was developed to determine the integral assessment of pollen quality, including a comprehensive analysis of its histochemical and morphological features characterizing viability. The sizes of J. deltoides pollen grains in Mountainous Crimea differ within the error limits. The average diameter of fertile pollen for the species, under the conditions of the peninsula, is 27.08 ± 0.49 μm. The average percentage of fertile (colored) pollen is 55.19 ± 3.84%. At the beginning of the 21st century, this figure ranged from 87.1% to 98.2%. It can be assumed that within the past two decades, the amount of fertile pollen has decreased by 1.7 times. The greatest influence on the fertility of J. deltoides pollen is almost equally exerted by the altitude above sea level and the edaphic conditions of the places of growth of individuals (6.38% and 6.50%, respectively). During the integral assessment of the pollen quality of the Crimean population of J. deltoides, it was found that the overwhelming majority of individuals (64.71%) are characterized by good quality pollen grains. The proportion of germinated pollen of J. deltoides in Mountainous Crimea is low and amounts to 20.16±1.12%. The main factor influencing the viability of pollen grains is the anthropogenic load of the territory (the strength of influence is 51.63%). The average proportion of germinated pollen in significantly anthropogenically disturbed areas is 12.0±1.26%, which is 2.3 times lower than that of individuals growing in undisturbed areas.
pyl'ca, J. deltoides, fertil'nost', zhiznesposobnost', kachestvo pyl'cy, faktory okruzhayuschey sredy, Gornyy Krym
1. Geodakyan V.A. Kolichestvo pyl'cy kak pokazatel' evolyucionnoy plastichnosti perekrestnoopylyayuschihsya rasteniy // Doklady Akademii nauk SSSR. 1977. T. 234 (6). S. 1460–1463.
2. Glotov N.V. Ocenka geneticheskoy geterogennosti prirodnyh populyaciy: kolichestvennye priznaki // Ekologiya. 1983. № 1. S. 3–10.
3. Dzyuba O.F., Kochubey O.V. Kachestvo pyl'cy rasteniy kak indikator intensivnosti vozdeystviya neftegazovogo kompleksa na prirodnuyu sredu ohranyaemyh territoriy Rossii // Neftegazovaya geologiya. Teoriya i praktika. 2014. T. 9. № 4. S. 1–24.
4. Isikov V.P., Plugatar' Yu.V., Koba V.P. Metody issledovaniy lesnyh ekosistem Kryma. Simferopol': IT «ARIAL», 2014. 252 s.
5. Koren'kova O.O. Biometricheskie pokazateli, dobrokachestvennost' i zhiznesposobnost' pyl'cy mozhzhevel'nika vysokogo (Juniperus excelsa M. Bieb.) v usloviyah Gornogo Kryma // Vestnik Orenburgskogo gosudarstvennogo pedagogicheskogo universiteta. Elektronnyy nauchnyy zhurnal. 2024. № 1(49). S. 32–45. https://doi.org/10.32516/2303-9922.2024.49.3.
6. Korsakova S.P., Sarkina I.S., Bagrikova N.A. Biologiya opyleniya Juniperus excelsa i J. deltoides (Cupressaceae) na Yuzhnom beregu Kryma // Botanicheskiy zhurnal. 2019. T. 104. № 10. S. 1574–1587. https://doi.org/10.1134/S0006813619100077.
7. Kotelova N.V. Proraschivanie pyl'cy na iskusstvennyh sredah i sposoby hraneniya pyl'cy sosny obyknovennoy // Nauchno-tehnicheskaya informaciya MLTI. 1956. № 23. S. 13–20.
8. Krasnaya kniga goroda Sevastopolya. Kaliningrad; Sevastopol': ID «ROST-DOAFK», 2018. 432 s.
9. Krasnaya kniga Respubliki Krym. Rasteniya, vodorosli i griby / otv. red. d.b.n., prof. A.V. Ena i k.b.n. A.V. Fateryga. Simferopol': IT «ARIAL», 2015. 480 s.
10. Lakin G.F. Biometriya. M.: Vysshaya shkola, 1990. 350 s.
11. Magomedmirzaev M.M. Vvedenie v kolichestvennuyu morfogenetiku. M.: Nauka, 1990. 230 s.
12. Mamaev S.A. Formy vnutrividovoy izmenchivosti drevesnyh rasteniy. M.: Nauka, 1973. 284 s.
13. Pausheva Z.P. Praktikum po citologii rasteniy. M.: Agropromizdat, 1988. 271 s.
14. Plugatar' Yu.V., Koren'kova O.O. Osobennosti vozrastnoy struktury mozhzhevelovyh drevostoev (Juniperus deltoides R. P. Adams, Juniperus excelsa M.-Bieb.) Kryma // Vestnik Orenburgskogo gosudarstvennogo pedagogicheskogo universiteta. Elektronnyy nauchnyy zhurnal. 2024. № 4(52). S. 26–37. https://doi.org/10.32516/2303-9922.2024.52.2.
15. Ruguzova A.I. Biologicheskie osobennosti mozhzhevel'nika krasnogo (Juniper oxycedrus L.) v Krymu v svyazi s ego ohranoy: avtoref. dis. … kand. biol. nauk. Yalta, 2006. 18 s.
16. Seroglazova N.G., Baktasheva N.M. Indikaciya chistoty okruzhayuschey sredy po sostoyaniyu pyl'cy rasteniy, proizrastayuschih v del'te r. Volgi // Vestn. MGOU. Ser. Estestvennye nauki. 2012. № 1. S. 65–68.
17. Surso M.V. Adaptaciya muzhskoy reproduktivnoy sfery mozhzhevel'nika obyknovennogo k klimatu // Izvestiya vysshih uchebnyh zavedeniy. Lesnoy zhurnal. 2018. № 6 (366). S. 57–69. https://doi.org/10.17238/issn0536-1036.2018.6.57.
18. Surso M.V., Selivanova N.V. Opylenie u mozhzhevel'nika obyknovennogo (Juniperus communis L.): mehanizm vovlecheniya pyl'cy i vliyanie muzhskogo gametofita na razvitie semyazachatkov i «shishkoyagod» // Izvestiya vysshih uchebnyh zavedeniy. Lesnoy zhurnal. 2016. № 4 (352). S. 40–53. https://doi.org/10.17238/issn0536-1036.2016.4.40.
19. Surso M.V., Chuhchin D.G., Hviyuzov S.S., Pokryshkin S.A. Mehanizm prorastaniya pyl'cy i rost pyl'cevyh trubok u mozhzhevel'nika obyknovennogo (Juniperus communis L.) in vitro // Ontogenez. 2020. T. 51. № 5. S. 351–362. https://doi.org/https://doi.org/10.31857/S0475145020050079.
20. Barnes C.S., Dhar M., Portnoy J.M. Impact Of Temperature And Rainfall On Airborne Juniper Pollen // Journal of Allergy and Clinical Immunology. 2011. Vol. 127. Iss. 2. P. AB95. https://doi.org/10.1016/j.jaci.2010.12.384.
21. Carmona-Velasco J., García-Cervigón A.I., Casimiro-Soriguer R. Population and reproductive structure of an endangered juniper from coastal pine forests // Journal for Nature Conservation. 2022. Vol. 67. P.126178. https://doi.org/10.1016/j.jnc.2022.126178.
22. Christopoulou A., Sazeides C.I., Fyllas N.M. Size-mediated effects of climate on tree growth and mortality in Mediterranean Brutia pine forests // Science of The Total Environment. 2022. Vol. 812. Art. 151463. https://doi.org/10.1016/j.scitotenv.2021.151463.
23. Dias M.C., Oliveira J.M.P.F., Marum L., Pereira V., Almeida T., Nunes S., Araújo M., Moutinho-Pereira J., Correia C. M., Santos C. Pinus elliottii and P. elliottii × P. caribaea hybrid differently cope with combined drought and heat episodes // Industrial Crops and Products. 2022. Vol. 176. Art. 114428. https://doi.org/10.1016/j.indcrop.2021.114428.
24. Gross L., Weber R., Wolf M., Crooks J. L. The impact of weather and climate on pollen concentrations in Denver, Colorado, 2010-2018 // Annals of Allergy, Asthma & Immunology. 2019. Vol. 123. Iss. 5, P. 494–502.e4. https://doi.org/10.1016/j.anai.2019.08.002.
25. Jiménez-Moreno G., Anderson R.S., Markgraf V., Staley S.E., Fawcett P.J. Environmental and climate evolution in the Southwest USA since the last interglacial deduced from the pollen record from Stoneman lake, Arizona // Quaternary Science Reviews. 2023. Vol. 300. P. 107883. https://doi.org/10.1016/j.quascirev.2022.107883.
26. Krichevsky A., Kozlovsky S.V., Tian G.-W., Chen M.-H., Zaltsman A., Citovsky V. How Pollen Tubes Grow (Review) // Developmental Biology. 2007. Vol. 303. P. 405–420. https://doi.org/10.1016/j.ydbio.2006.12.003.
27. López-Orozco R., García-Mozo H., Oteros J., Galán C. Long-term trends in atmospheric Quercus pollen related to climate change in southern Spain: A 25-year perspective // Atmospheric Environment. 2021. Vol. 262. Art. 118637. https://doi.org/10.1016/j.atmosenv.2021.118637.
28. Manangan A., Brown C., Saha Sh., Bell J., Hess J., Uejio Ch., Fineman S., Schramm P. Long-term pollen trends and associations between pollen phenology and seasonal climate in Atlanta, Georgia (1992-2018) // Annals of Allergy, Asthma & Immunology. 2021. Vol. 127. Iss. 4. P. 471–480.e4. https://doi.org/10.1016/j.anai.2021.07.012.
29. Muldavin E., Triepke F.J. North American Pinyon – Juniper Woodlands: Ecological Composition, Dynamics, and Future Trends // Encyclopedia of the World's Biomes. 2020. P. 516–531. https://doi.org/10.1016/B978-0-12-409548-9.12113-X.
30. Vergotti M.J., Fernández-Martínez M., Kefauver S.C., Janssens I.A., Penuelas J. Weather and trade-offs between growth and reproduction regulate fruit production in European forests // Agricultural and Forest Meteorology. 2019. Vol. 279. Art. 107711. https://doi.org/https://doi.org/10.1016/j.agrformet.2019.107711.
