Ekaterinburg, Russian Federation
Ekaterinburg, Russian Federation
Ekaterinburg, Russian Federation
Ekaterinburg, Russian Federation
The deficiency of essential micronutrients, particularly iodine, poses a serious threat to public health. In this regard, the search for effective methods of biofortification of plant materials, including the use of plant growth-promoting (PGP) rhizobacteria, is a relevant task. The aim of this study was to evaluate the PGP-activity of three Pseudomonas sp. strains (CTF2, CTF7, STF13), as well as their effect on growth parameters, photosynthetic pigment content, and iodine accumulation in Vigna radiata (L.) R. Wilczek (mung bean) microgreens in combination with foliar treatment with potassium iodide and potassium iodate solutions. The strains were isolated from the rhizosphere of Tussilago farfara growing in technogenically disturbed areas. A vegetation experiment was conducted using a hydroponic system with seed inoculation followed by iodine treatment of the plants. It was found that strain STF13 exhibited the highest ability to synthesize indole-3-acetic acid and the highest 1-aminocyclopropane-1-carboxylate deaminase activity. Inoculation of seeds with this strain led to the greatest increases in fresh and dry biomass of 14-day-old seedlings, as well as in their growth vigor index. All studied strains increased the content of chlorophylls and carotenoids by an average of 19% compared to the control. Foliar treatment with iodine significantly increased the content of photosynthetic pigments (by 23% on average) and promoted the accumulation of iodine in microgreens: when using KI, the content of the element increased by 14.3 times, KIO₃ – by 8.8 times. Thus, the combination of inoculating V. radiata seeds with promising Pseudomonas sp. strains (especially STF13 and CTF2) together with foliar iodine application increases the biological value of microgreens, confirming the effectiveness of this approach.
mung bean, biofortification, plant growth-promoting rhizobacteria, growth parameters, photosynthetic pigments, potassium iodide and potassium iodate, iodine accumulation
1. Borisova, G.G., Maleva, M.G., Darkazanli, M., Sobenin, A.V., & Karpuxin, M.Yu. (2004). Vliyanie Zn-solyubiliziruyushhix PGP-rizobakterij na rost i soderzhanie biogenny`x e`lementov v seyanczax pshenicy ozimoj v usloviyax mikrokosma. Agrarny`j vestnik Urala, 24(12), 1636-1647. (in Russ.). https://doi.org/10.32417/1997-4868-2024-24-12-1636-1647
2. Golubkina, N.A., Kekina, E.G., & Nadezhkin, S.M. (2015). Perspektivy` obogashheniya sel`skoxozyajstvenny`x rastenij jodom i selenom (obzor). Mikroe`lementy` v medicine, 16(3), 12-19. (in Russ.).
3. GOST 28458-90. Rastitel`ny`e korma, Sposob opredeleniya joda. Moskva: Standartinform, 2006. (in Russ.).
4. Eliseeva, L.G., Belkin, Yu.D., Simina, D.V., Osman, A., Molodkina, P.G., & Santuryan, T.A. (2022). Novy`e napravleniya razrabotki obogashhenny`x pishhevy`x produktov dlya zdorovogo pitaniya. Mezhdunarodny`j nauchno-issledovatel`skij zhurnal, 4(118), 50-55. (in Russ.). https://doi.org/10.23670/IRJ.2022.118.4.009
5. Eliseeva, L.G., Simina, D.V., Sidorenko, Yu.I., Tokarev, P.I., & Santuryan, T.A. (2024). Razrabotka podxodov biofortifikacii mikrozeleni pri vy`rashhivanii v fitotrone gorodskogo tipa. Vestnik Voronezhskogo gosudarstvennogo universiteta inzhenerny`x texnologij, 86(4), 84-92. (in Russ.). https://doi.org/10.20914/2310-1202-2024-4-84-92
6. Kur`yanovich, A.A., Kincharova, M.N., & Titova, I.A. (2021). Vy`rashhivanie prorostkov masha (Vigna radiata l.(R) Wilczek) dlya pishhevy`x celej. Vestnik Ul`yanovskoj gosudarstvennoj sel`skoxozyajstvennoj akademii, 4(56), 25-30. (in Russ.). https://doi.org/10.18286/1816-4501-2021-4-25-30
7. Platonova, N.M. (2015). Jodny`j deficit: sovremennoe sostoyanie problemy` (2015). Klinicheskaya i e`ksperimental`naya tireoidologiya, 11(1), 12-21. (in Russ.). https://doi.org/https://doi.org/10.14341/ket2015112-21
8. Timofeeva, A.M., Galyamova, M.R., & Sedy`x, S.E. (2024). Biologicheskaya aktivnost` pochvenny`x bakterij, stimuliruyushhix rost rastenij: fiksaciya azota, solyubilizaciya fosfata, sintez sideroforov. Perspektivy` razrabotki mikrobny`x konsorciumov. Agroximiya, 5, 85-95. (in Russ.). https://doi.org/10.31857/S0002188124050111
9. Ciriello, M., Formisano, L., El-Nakhel, C., Zarrelli, A., Giordano, M., De Pascale, S., Kyriacou, M., & Rouphael, Y. (2023). Iodine biofortification of four microgreens species and its implications for mineral composition and potential contribution to the recommended dietary intake of iodine. Scientia Horticulturae, 320, 112229. https://doi.org/10.1016/j.scienta.2023.112229
10. Consentino, B.B., Ciriello, M., Sabatino, L., Vultaggio, L., Baldassano, S., Vasto, S., Rouphael, Y., La Bella, S., & De Pascale, S. (2023). Current acquaintance on agronomic biofortification to modulate the yield and functional value of vegetable crops: A review. Horticulturae, 9, 219. https://doi.org/10.3390/horticulturae9020219
11. Dhaliwal, S.S., Sharma, V., Shukla, A., Kaur, M., Kaur, J., Verma, V., Singh, P., Barek, V., Gaber, A., & Hossain, A. (2023). Biofortification of mungbean (Vigna radiata L. (Wilczek)) with boron, zinc and iron alters its grain yield and nutrition. Scientific Reports, 13, 3506. https://doi.org/10.1038/s41598-023-30539-6
12. Dobosy, P., Nguyen, H.T.P., Záray, G., Streli, C., Ingerle, D., Ziegler, P., Radtke, M., Buzanich, A.G., Endrédi, A., & Fodor, F. (2024). Effect of iodine species on biofortification of iodine in cabbage plants cultivated in hydroponic cultures. Scientific Reports, 14(1), 15794. https://doi.org/10.1038/s41598-024-66575-z
13. Golubkina, N., Moldovan, A., Kekina, H., Kharchenko, V., Sekara, A., Vasileva, V., Skrypnik, L., Tallarita, A., & Caruso, G. (2021). Joint biofortification of plants with selenium and iodine. New field of discoveries. Plants, 10, 1352. https://doi.org /10.3390/plants10071352
14. Kiferle, C., Martinelli, M., Salzano, A.M., Gonzali, S., Beltrami, S., Salvadori, P.A., Hora, K., Holwerda, H.T., Scaloni, A., & Perata, P. (2021). Evidences for a nutritional role of iodine in plants. Frontiers in Plant Science, 12, 671-683. https://doi.org/10.3389/fpls.2021.616868
15. Khan, S., Ullah, A., Ullah, S., Saleem, M.H., Okla, M.K., Al-Hashimi, A., Chen, Y., & Ali, S. (2022) Quantifying temperature and osmotic stress impact on seed germination rate and seedling growth of Eruca sativa Mill. via hydrothermal time model. Life, 12, 400. https://doi.org/10.3390/life12030400
16. Kumar, A., Tripti, Voropaeva, O., Maleva, M., Panikovskaya, K., Borisova, G., Rajkumar, M., & Bruno, L.B. (2021). Bioaugmentation with copper tolerant endophyte Pseudomonas lurida strain EOO26 for improved plant growth and copper phytoremediation by Helianthus annuus. Chemosphere, 266, 128983. https://doi.org/10.1016/j.chemosphere.2020.128983
17. Lichtenthaler, H.K. (1987). Chlorophylls and carotenoids: pigments of photosynthetic membranes. Methods in Enzymology, 148, 350-382. https://doi.org/10.1016/0076-6879(87)48036-1
18. Maleva, M., Borisova, G., Tripti, Tugbaeva, A., Ahamuefule, C., Salata, A., & Kumar, A. (2024). Biofortification of pea microgreens through zinc-solubilizing bacteria inoculation with foliar iodine application. Agriculture and Forest, 70(2), 123-134. https://doi.org/10.17707/AgricultForest.70.2.9
19. Maleva, M., Borisova, G., Tugbaeva, A., Ilyin, V., & Kumar, A. (2024). Synergy of new zinc solubilizing PGPR strain Pseudomonas sp. CTF2 with foliar iodine application enhances growth and biofortification of pea seedlings. Microbiology, 93(1), S82-S86. https://doi.org/10.1134/S0026261724609333
20. Medrano-Macías, J., Leija-Martínez, P., González-Morales, S., Juárez-Maldonado, A., & Benavides-Mendoza, A. (2016). Use of iodine to biofortify and promote growth and stress tolerance in crops. Frontiers in Plant Science, 7, 1146. https://doi.org/10.3389/fpls.2016.01146
21. Şahin, Ö. (2020). Combined iodine, iron and zinc biofortification of tomato fruit. Journal of the Institute of Science and Technology, 10(3), 2242-2251. https://doi.org/10.21597/jist.691758
22. Voogt, W., Holwerda, H.T., & Khodabaks, R. (2010). Biofortification of lettuce (Lactuca sativa L.) with iodine: the effect of iodine form and concentration in the nutrient solution on growth, development and iodine uptake of lettuce grown in water culture (2010). Journal of the Science of Food and Agriculture, 90(5), 906-913. https://doi.org/10.1002/jsfa.3902
