Expression of Long Non-Coding RNAs MALAT1, GAS5, TUG1 in Peripheral Blood Leucocytes of Pulmonary Sarcoidosis Patients Before and After Glucocorticosteroid Therapy

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Abstract

The expression levels of long non-coding RNAs (lncRNA) MALAT1, GAS5, TUG1 in peripheral blood leukocytes (PBL) of patients with pulmonary sarcoidosis (n = 30, mean age 47 ± 5.23 years) and conditionally healthy donors control group (n = 27, mean age 42 ± 3.65 years) was analyzed. The expression level of lncRNAs MALAT1, GAS5, TUG1 was determined in PBLs by real-time PCR/ According to the obtained data in patients with progressive pulmonary sarcoidosis (stage II of the disease) the expression level of lncRNAs MALAT1, GAS5, TUG1 was significantly increased compared to the control group (p < 0.001). Under the glucocorticosteroid (GCS) therapy, a decrease level of lncRNAs MALAT1 and TUG1 was detected, while the expression of GAS5 increased before and during GCS therapy (p < 0.001). The observed changes in the expression level of the studied long non-coding RNAs may be associated both with the development of the inflammatory process in pulmonary sarcoidosis, and with the pathogenetic mechanisms of this disease.

About the authors

I. E. Malysheva

Institute of Biology of the Karelian Research Centre, Russian Academy of Sciences

Author for correspondence.
Email: i.e.malysheva@yandex.ru
Petrozavodsk, 185910 Russia

O. V. Balan

Institute of Biology of the Karelian Research Centre, Russian Academy of Sciences

Email: i.e.malysheva@yandex.ru
Petrozavodsk, 185910 Russia

E. L. Tikhonovich

Republican Hospital named after V.A. Baranov

Email: i.e.malysheva@yandex.ru
Petrozavodsk, 185019 Russia

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