Determination of the level of food contamination with Aspergillus flavus and aflatoxin B1 in the territory of the Adygeya Republic

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Investigations were executed to determine the level of the food contamination with mold fungi, to identify A.flavus and to assess the concentration of aflatoxin B1. Culturing is performed on Capek culture medium, A.flavus is identified according to L.I. Kursanov, the qualitative estimation of aflatoxin B1 in liquid culture and food products was carried out with the use of thin-layer chromatography method on “Silufol” UV-254 plates, and quantification was made by high-performance liquid chromatography (Agilent Technologies 1200 series). Out of 17 investigated samples, 23.5% were established to be mold fungi-contaminated, of which 11.3% were contaminated with A. flavus. In one sample, isolated from A. flavus toxigenic strain, aflatoxin B1 was detected in the concentration of 0.0072 ± 0.00028 mg/kg.

Sobre autores

Aminet Tsikunib

Adyghea State University

Autor responsável pela correspondência
Email: cikunib58@mail.ru
ORCID ID: 0000-0002-7491-0539

MD, Ph.D., DSci., professor, Head of the Department of Chemistry of the Adyghea State University, Director of the Institute for Complex Problems of the Adyghea State University, Maykop, 385000, Russian Federation. 

e-mail: cikunib58@mail.ru

Rússia

I. Grin

Adyghea State University

Email: noemail@neicon.ru
ORCID ID: 0000-0001-5690-2407
Rússia

Bibliografia

  1. Afonyushkin V.N., Leonov S.V., Gorodov V.S., Morozov K.V., Dudareva E.V. Mikotoksikozy: znachenie, diagnostika, bor’ba. Arkhiv veterinarnykh nauk. 2005; 6(53): 27-34. (in Russian)
  2. Bilay V.I., Podoplichenko N.M. Aspergillotoksikozy. In: Bilay V.I., Podoplichenko N.M. Toksinoobrazujushhie mikroskopicheskie griby. Kiev: Naukova dumka; 1970: 104-29. (in Russian)
  3. Bilay V.I., Podoplichenko N.M. Toksinoobrazujushhie mikroskopicheskie griby. Kiev: Naukova dumka; 1970. (in Russian)
  4. Grigor’eva R.Z. Bezopasnost’ prodovol’stvennogo syr’ya i produktov pitaniya. Uchebnoe posobie. Kemerovo: KemTIPP; 2004. (in Russian)
  5. Kursanov L.I. Posobie po opredeleniyu gribov iz rodov Aspergillus i Penicillium. Moscow: Medgiz; 1947. (in Russian)
  6. Kononenko G.P. Fuzariotoksiny v zerne kolosovykh kul’tur: regional’nye osobennosti. In: Kononenko G.P., Burkin A.A. Uspekhi meditsinskoy mikologii. t. 1. Moscow: Natsional’naya akademiya mikologii; 2003: 141-4. (in Russian)
  7. Monastyrskiy O.A. Toksinoobrazuyushchie griby, parazitiruyushchie na zerne. Agro XXI. 2001; (11): 6-7. (in Russian)
  8. Mayanskiy A.N. Vvedenie v meditsinskuyu mikologiyu: uchebno-metodicheskoe posobie. N. Novgorod: NGMA; 2000. (in Russian)
  9. Bilay V.I. Metody eksperimental’noy mikologii: spravochnik. Kiev: Naukova dumka; 1982: 309-12. (in Russian)
  10. Smirnova I.R., Mikhalev A.V., Satyukova L.P., Borisova V.S. Sovremennoe sostoyanie kachestva i bezopasnosti kormov v Rossii. Veterinariya. 2009; (2): 3-7. (in Russian)
  11. Tutel’yan V.A., Kravchenko L.V. Mikotoksiny (meditsinskie i biologicheskie aspekty). Moscow: Meditsina; 1985. (in Russian)
  12. Bakheet S.A., Alhuraishi A.M., Al-Harbi N.O., Al-Hosaini K.A., Al-Sharary S.D., Attia M.M. et al. Alleviation of aflatoxin B1-induced genomic damage by proanthocyanidins via modulation of DNA repair. J. Biochem. Mol. Toxicol. 2016; 30(11): 559-66. https://doi.org/10.1002/jbt.21823
  13. Ghufran M., Ghosh K., Kanade S.R. Aflatoxin B1 induced upregulation of protein arginine methyltransferase 5 in human cell lines. Toxicon. 2016; 119: 117-21. https://doi.org/10.1016/j.toxicon.2016.05.015
  14. Gupta D., Sharma Y.P., Bala P. Mycoflora and natural aflatoxin contamination in dried quince seeds from Jammu, India. J. Environ. Biol. 2016; 37(1): 101-6.
  15. Food and agriculture organization of the United Nations. Available at: http://www.fao.org
  16. ВОЗ/Всемирная организация здравоохранения. Available at: http://www.who.int.ru (in Russian)
  17. http://www.webofknowledge.com

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