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Methods for the Detection of Muscarinic, Muscimol and Ibotenic Acid in Fungi and Biological Matrices Using the HPLC-MS/MS Method
- Authors: Aigumov M.S.1, Novikov A.P.2, Vishnevskii M.V.3, Chernova N.A.2, Maidanec I.V.2, Gofenberg M.A.4, Kuznecov D.V.5, Samyshkina N.V.6, Rizvanova L.N.7, Temerdashev A.Z.8, Savchuk S.A.9,10
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Affiliations:
- Noyabrsk Psychoneurological Dispensary
- Surgut Clinical Psychoneurological Hospital
- Center for Innovative Mycological Research
- Sverdlovsk Regional Clinical Psychiatric Hospital
- Volgograd Regional Clinical Narcological Dispensary
- Novourengoy Psychoneurological Dispensary
- Nizhnevartovsk Psychoneurological Dispensary
- Kuban State University
- Association of Specialists in Chemical-Toxicological and Forensic Chemical Analysis
- The Institute of Physical Chemistry and Electrochemistry RAS (IPCE RAS)
- Issue: Vol 80, No 2 (2025)
- Pages: 161-173
- Section: ORIGINAL ARTICLES
- Submitted: 13.06.2025
- URL: https://vestnik.nvsu.ru/0044-4502/article/view/684152
- DOI: https://doi.org/10.31857/S0044450225020056
- EDN: https://elibrary.ru/adybim
- ID: 684152
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Abstract
An algorithm for two-step detection of muscarinic, ibotenic acid and muscimol in urine samples is described. A two-step method for the detection of muscarinic, muscimol and ibotenic acid in data-dependent MRM analysis mode with simultaneous registration of complete mass spectra of the target substances was developed. Detection of analytes was carried out by HPLC with a three-quadrupole mass spectrometric detector (LC-MS/MS Shimadzu 8050). Chromatographic separation was performed on a column with reversed-phase sorbent Phenomenex Kinetex C18. At the first stage, muscarine was detected in a diluted urine sample; at the second stage, muscimol and ibotenic acid were detected using the bimolecular dansylation method. The method was successfully applied in practice for routine chemical-toxicological studies of urine samples of patients delivered with suspected mushroom poisoning.
About the authors
M. Sh. Aigumov
Noyabrsk Psychoneurological Dispensary
Author for correspondence.
Email: aygumov.m@yandex.ru
Russian Federation, Noyabrsk
A. P. Novikov
Surgut Clinical Psychoneurological Hospital
Email: aygumov.m@yandex.ru
Russian Federation, Surgut
M. V. Vishnevskii
Center for Innovative Mycological Research
Email: aygumov.m@yandex.ru
Russian Federation, Moscow
N. A. Chernova
Surgut Clinical Psychoneurological Hospital
Email: aygumov.m@yandex.ru
Russian Federation, Surgut
I. V. Maidanec
Surgut Clinical Psychoneurological Hospital
Email: aygumov.m@yandex.ru
Russian Federation, Surgut
M. A. Gofenberg
Sverdlovsk Regional Clinical Psychiatric Hospital
Email: aygumov.m@yandex.ru
Russian Federation, Ekaterinburg
D. V. Kuznecov
Volgograd Regional Clinical Narcological Dispensary
Email: aygumov.m@yandex.ru
Russian Federation, Volgograd
N. V. Samyshkina
Novourengoy Psychoneurological Dispensary
Email: aygumov.m@yandex.ru
Russian Federation, Novy Urengoy
L. N. Rizvanova
Nizhnevartovsk Psychoneurological Dispensary
Email: aygumov.m@yandex.ru
Russian Federation, Nizhnevartovsk
A. Z. Temerdashev
Kuban State University
Email: aygumov.m@yandex.ru
Educational, scientific and production team of the Scientific and Production Complex “Analit”
Russian Federation, KrasnodarS. A. Savchuk
Association of Specialists in Chemical-Toxicological and Forensic Chemical Analysis; The Institute of Physical Chemistry and Electrochemistry RAS (IPCE RAS)
Email: aygumov.m@yandex.ru
Russian Federation, St. Petersburg; Moscow
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