Methods for the Detection of Muscarinic, Muscimol and Ibotenic Acid in Fungi and Biological Matrices Using the HPLC-MS/MS Method

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An algorithm for two-step detection of muscarinic, ibotenic acid and muscimol in urine samples is described. A two-step method for the detection of muscarinic, muscimol and ibotenic acid in data-dependent MRM analysis mode with simultaneous registration of complete mass spectra of the target substances was developed. Detection of analytes was carried out by HPLC with a three-quadrupole mass spectrometric detector (LC-MS/MS Shimadzu 8050). Chromatographic separation was performed on a column with reversed-phase sorbent Phenomenex Kinetex C18. At the first stage, muscarine was detected in a diluted urine sample; at the second stage, muscimol and ibotenic acid were detected using the bimolecular dansylation method. The method was successfully applied in practice for routine chemical-toxicological studies of urine samples of patients delivered with suspected mushroom poisoning.

作者简介

M. Aigumov

Noyabrsk Psychoneurological Dispensary

编辑信件的主要联系方式.
Email: aygumov.m@yandex.ru
俄罗斯联邦, Noyabrsk

A. Novikov

Surgut Clinical Psychoneurological Hospital

Email: aygumov.m@yandex.ru
俄罗斯联邦, Surgut

M. Vishnevskii

Center for Innovative Mycological Research

Email: aygumov.m@yandex.ru
俄罗斯联邦, Moscow

N. Chernova

Surgut Clinical Psychoneurological Hospital

Email: aygumov.m@yandex.ru
俄罗斯联邦, Surgut

I. Maidanec

Surgut Clinical Psychoneurological Hospital

Email: aygumov.m@yandex.ru
俄罗斯联邦, Surgut

M. Gofenberg

Sverdlovsk Regional Clinical Psychiatric Hospital

Email: aygumov.m@yandex.ru
俄罗斯联邦, Ekaterinburg

D. Kuznecov

Volgograd Regional Clinical Narcological Dispensary

Email: aygumov.m@yandex.ru
俄罗斯联邦, Volgograd

N. Samyshkina

Novourengoy Psychoneurological Dispensary

Email: aygumov.m@yandex.ru
俄罗斯联邦, Novy Urengoy

L. Rizvanova

Nizhnevartovsk Psychoneurological Dispensary

Email: aygumov.m@yandex.ru
俄罗斯联邦, Nizhnevartovsk

A. Temerdashev

Kuban State University

Email: aygumov.m@yandex.ru

Educational, scientific and production team of the Scientific and Production Complex “Analit”

俄罗斯联邦, Krasnodar

S. Savchuk

Association of Specialists in Chemical-Toxicological and Forensic Chemical Analysis; The Institute of Physical Chemistry and Electrochemistry RAS (IPCE RAS)

Email: aygumov.m@yandex.ru
俄罗斯联邦, St. Petersburg; Moscow

参考

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  8. Tsujikawa K., Kuwayama K., Miyaguchi H., Kanamori T., Iwata Y., Inoue H. et al. Determination of muscimol and ibotenic acid in Amanita mushrooms by high-performance liquid chromatography and liquid chromatography-tandem mass spectrometry // J. Chromatogr. B: Anal. Technol. Biomed. Life Sci. 2007. V. 852. № 2. P. 430.
  9. Xu X.M., Zhang J.S., Huang B.F., Han J.L., Chen Q. Determination of ibotenic acid and muscimol in plasma by liquid chromatography-triple quadrupole mass spectrometry with bimolecular dansylation // J. Chromatogr. B: Anal. Technol. Biomed. Life Sci. 2020. V. 1146. P. 122. https://doi.org/10.1016/j.jchromb.2020.122128

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