ANATOMICAL, MORPHOLOGICAL, AND BIOCHEMICAL CHARACTERISTICS OF DIFFERENT-AGED NEEDLES OF PINUS SIBIRICA DU TOUR (MIDDLE URALS)

Nadezhda Chukina; Nataliya Lukina; Elena I. Filimonova; Margarita A. Glazyrina

doi:10.36906/2311-4444/25-4/01

ANATOMICAL, MORPHOLOGICAL, AND BIOCHEMICAL CHARACTERISTICS OF DIFFERENT-AGED NEEDLES OF PINUS SIBIRICA DU TOUR (MIDDLE URALS)

Published in Bulletin of Nizhnevartovsk State University · Pages 4–15 · Rubric: Plant Ecology

DOI: https://doi.org/10.36906/2311-4444/25-4/01

Received: 25.09.2025 Accepted: 29.10.2025 Published: 25.12.2025

Authors

Nadezhda Chukina

ORCID

Nataliya Lukina

ORCID

Elena I. Filimonova

ORCID

Margarita A. Glazyrina

ORCID

Nadezhda Chukina

Academic degree: candidate of sciences in biology

Ural Federal University named after the First President of Russia B.N. Yeltsin Russian Federation · Yekaterinburg

ORCID

Nataliya Lukina

Academic degree: candidate of sciences in biology

Ural Federal University named after the First President of Russia B.N. Yeltsin Russian Federation · Yekaterinburg

ORCID

Elena I. Filimonova

Academic degree: candidate of sciences in biology

Ural Federal University named after the First President of Russia B.N. Yeltsin Russian Federation · Yekaterinburg

ORCID

Margarita A. Glazyrina

Academic degree: candidate of sciences in biology

Ural Federal University named after the First President of Russia B.N. Yeltsin Russian Federation · Yekaterinburg

ORCID

ABSTRACT

The aim of the present study was to investigate the anatomical, morphological, and biochemical characteristics of Pinus sibirica Du Tour needles of different ages, growing in their natural habitat in the taiga zone (Middle Urals). Anatomical and morphological parameters of the needles were analyzed in cross-sections using the «SIAMS MesoPlant» image processing system. The content of lowmolecular antioxidants, such as proline, ascorbic acid, phenolic compounds, including flavonoids, and the level of lipid peroxidation (LPO) in the needles were analyzed using standard spectrophotometric methods. It was shown that with the needle aging a decrease in anatomical and morphological parameters such as cross-sectional area, mesophyll area, and central cylinder was observed. The thickness of the covering tissues (epidermis and hypodermis) did not change significantly with the needle aging. It was found the decrease of green pigments concentration in needles due to a significant reduction in chlorophyll a, as well as a decrease in the ratio of chlorophyll a to antenna pigments with the needle aging. The content of carotenoids and chlorophyll b in the needles did not change significantly, indicating the stability of antenna pigments pool. A significant increase in lipid peroxidation was observed in 4-year-old needles, as a result, th antioxidant defense system was activated, manifested by an increase in the phenolic compounds and free proline content. The accumulation of ascorbic acid decreased with the needle aging.

KEYWORDS

Pinus sibirica; needles anatomy; photosynthetic pigments; lipid peroxidation; low-molecular antioxidants.

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GOST

Chukina N. V., Lukina N. V., Filimonova E., Glazyrina M. ANATOMICAL, MORPHOLOGICAL, AND BIOCHEMICAL CHARACTERISTICS OF DIFFERENT-AGED NEEDLES OF PINUS SIBIRICA DU TOUR (MIDDLE URALS) // . 2025. pp. 4-15. DOI: https://doi.org/10.36906/2311-4444/25-4/01. (Date of access 19.08.2026).

MLA

Nadezhda Chukina, Lukina N. V., Filimonova E., Glazyrina M.. "ANATOMICAL, MORPHOLOGICAL, AND BIOCHEMICAL CHARACTERISTICS OF DIFFERENT-AGED NEEDLES OF PINUS SIBIRICA DU TOUR (MIDDLE URALS)." (2025): 4-15. DOI: https://doi.org/10.36906/2311-4444/25-4/01.

APA

Chukina N. V., Lukina N. V., Filimonova E., Glazyrina M. (2025). ANATOMICAL, MORPHOLOGICAL, AND BIOCHEMICAL CHARACTERISTICS OF DIFFERENT-AGED NEEDLES OF PINUS SIBIRICA DU TOUR (MIDDLE URALS). , 4-15. https://doi.org/10.36906/2311-4444/25-4/01

Chicago

Nadezhda Chukina, Lukina N. V., Filimonova E., Glazyrina M.. "ANATOMICAL, MORPHOLOGICAL, AND BIOCHEMICAL CHARACTERISTICS OF DIFFERENT-AGED NEEDLES OF PINUS SIBIRICA DU TOUR (MIDDLE URALS)." , no. (2025): 4-15. https://doi.org/10.36906/2311-4444/25-4/01.

WIKI

{{cite journal | author = Chukina N. V., Lukina N. V., Filimonova E., Glazyrina M. | title = ANATOMICAL, MORPHOLOGICAL, AND BIOCHEMICAL CHARACTERISTICS OF DIFFERENT-AGED NEEDLES OF PINUS SIBIRICA DU TOUR (MIDDLE URALS) | journal = | year = 2025 | pages = 4-15 | doi = 10.36906/2311-4444/25-4/01 }}

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ANATOMICAL, MORPHOLOGICAL, AND BIOCHEMICAL CHARACTERISTICS OF DIFFERENT-AGED NEEDLES OF PINUS SIBIRICA DU TOUR (MIDDLE URALS)

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