ANATOMICAL, MORPHOLOGICAL, AND BIOCHEMICAL CHARACTERISTICS OF THE ORCHID EPIPACTIS PALUSTRIS ON TECHNOGENIC SUBSTRATES

Nadezhda V. Chukina; Margarita A. Glazyrina; Elena I. Filimonova; Natalia V. Lukina; Anastasia A. Kordyuk

doi:10.36906/2311-4444/26-2/10

UDC

581.5

ANATOMICAL, MORPHOLOGICAL, AND BIOCHEMICAL CHARACTERISTICS OF THE ORCHID EPIPACTIS PALUSTRIS ON TECHNOGENIC SUBSTRATES

Published in Bulletin of Nizhnevartovsk State University · Pages 109–122 · Rubric: Plant Ecology

DOI: https://doi.org/10.36906/2311-4444/26-2/10

Received: 03.04.2026 Accepted: 08.06.2026 Published: 25.06.2026

Authors

Nadezhda V. Chukina

ORCID

Margarita A. Glazyrina

ORCID

Elena I. Filimonova

ORCID

Natalia V. Lukina

ORCID

Anastasia A. Kordyuk

Nadezhda V. Chukina

Academic degree: candidate of sciences in biology

Ural Federal University named First President of Russia B. Yeltsin

ORCID

Margarita A. Glazyrina

Academic degree: candidate of sciences in biology

Ural Federal University named after the First President of Russia B.N. Yeltsin

ORCID

Elena I. Filimonova

Academic degree: candidate of sciences in biology

Ural Federal University named after the First President of Russia B.N. Yeltsin Russian Federation · Yekaterinburg

ORCID

Natalia V. Lukina

Academic degree: candidate of sciences in biology

Ural Federal University named after the First President of Russia B.N. Yeltsin

ORCID

Anastasia A. Kordyuk

Ural Federal University named First President of Russia B. Yeltsin departament nauk o Zemle i kosmose

ABSTRACT

The aim of this study was to investigate the anatomical, morphological, and biochemical parameters of Epipactis palustris (L.) Crantz growing in plant communities formed on ash dumps of the Nizhneturinskaya and Reftinskaya state power stations and in a natural plant community. Anatomical and morphological parameters of leaves were studied using the SIAMS Mesoplant image processing system. The content of pigments and non-enzimatic antioxidants, as well as the level of lipid peroxidation in leaves, were analyzed using standard spectrophotometric methods. It was shown that in E. palustris growing on ash dumps, the height of generative shoots, leaf area, and the number of flowers decreased. Plants growing in transformed habitats accumulated less nitrogen and phosphorus in leaves due to deficiency of these elements in the ash substrates. On ash dumps, E. palustris exhibited an increase in the leaf internal assimilating surface, which is photosynthetic apparatus adaptive response to increased insolation and reduced substrate water availability. In the leaves of plants growing on ash dumps, the content of chlorophyll a and carotenoids was decreased. Under such unfavorable conditions, the content of non-enzymatic antioxidants (ascorbic acid, proline, and soluble phenol) was increased in E. palustris leaves. The results of this comprehensive study indicate that disturbed habitats (ash dumps) can serve as temporary habitats for the conservation of the gene pool of E. palustris.

KEYWORDS

Epipactis palustris, ash dump, coenopopulations, morphophysiological parameters, pigments, anti-oxidants

Funding

Ministry of Science and Higher Education of the Russian Federation (FEUZ-2026-0017)

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GOST

Chukina N., Glazyrina M., Filimonova E., Lukina N., Kordyuk A. ANATOMICAL, MORPHOLOGICAL, AND BIOCHEMICAL CHARACTERISTICS OF THE ORCHID EPIPACTIS PALUSTRIS ON TECHNOGENIC SUBSTRATES // . 2026. pp. 109-122. DOI: https://doi.org/10.36906/2311-4444/26-2/10. (Date of access 19.08.2026).

MLA

Nadezhda V. Chukina, Glazyrina M., Filimonova E., Lukina N., Kordyuk A.. "ANATOMICAL, MORPHOLOGICAL, AND BIOCHEMICAL CHARACTERISTICS OF THE ORCHID EPIPACTIS PALUSTRIS ON TECHNOGENIC SUBSTRATES." (2026): 109-122. DOI: https://doi.org/10.36906/2311-4444/26-2/10.

APA

Chukina N., Glazyrina M., Filimonova E., Lukina N., Kordyuk A. (2026). ANATOMICAL, MORPHOLOGICAL, AND BIOCHEMICAL CHARACTERISTICS OF THE ORCHID EPIPACTIS PALUSTRIS ON TECHNOGENIC SUBSTRATES. , 109-122. https://doi.org/10.36906/2311-4444/26-2/10

Chicago

Nadezhda V. Chukina, Glazyrina M., Filimonova E., Lukina N., Kordyuk A.. "ANATOMICAL, MORPHOLOGICAL, AND BIOCHEMICAL CHARACTERISTICS OF THE ORCHID EPIPACTIS PALUSTRIS ON TECHNOGENIC SUBSTRATES." , no. (2026): 109-122. https://doi.org/10.36906/2311-4444/26-2/10.

WIKI

{{cite journal | author = Chukina N., Glazyrina M., Filimonova E., Lukina N., Kordyuk A. | title = ANATOMICAL, MORPHOLOGICAL, AND BIOCHEMICAL CHARACTERISTICS OF THE ORCHID EPIPACTIS PALUSTRIS ON TECHNOGENIC SUBSTRATES | journal = | year = 2026 | pages = 109-122 | doi = 10.36906/2311-4444/26-2/10 }}

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ANATOMICAL, MORPHOLOGICAL, AND BIOCHEMICAL CHARACTERISTICS OF THE ORCHID EPIPACTIS PALUSTRIS ON TECHNOGENIC SUBSTRATES

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