ECOLOGICAL-GENETIC ANALYSIS OF SECALE CEREALE L. CULTIVARS GROWN IN CIS-URALS

Viktoriya A. Pechenkina

doi:10.36906/2311-4444/26-2/09

UDC

57.047

CSCSTI

34.35 34.23

Russian Classification of Professions by Education

06.00.00

Russian Trade and Bibliographic Classification

Russian Library and Bibliographic Classification

VAK Russia

1.5.15

ECOLOGICAL-GENETIC ANALYSIS OF SECALE CEREALE L. CULTIVARS GROWN IN CIS-URALS

Published in Bulletin of Nizhnevartovsk State University · Pages 98–108 · Rubric: Plant Ecology

DOI: https://doi.org/10.36906/2311-4444/26-2/09

Received: 07.04.2026 Accepted: 08.06.2026 Published: 25.06.2026

Authors

Viktoriya A. Pechenkina

ORCID Scopus ResearcherID Scholar SPIN

Viktoriya A. Pechenkina

Perm State University

Permskiy federal'nyy issledovatel'skiy centr Ural'skogo otdeleniya Rossiyskoy akademii nauk (PFIC UrO RAN) Russian Federation

ORCID Scopus ResearcherID Scholar SPIN

ABSTRACT

Ecological and genetic analysis of winter rye (Secale cereale L.) cultivars cultivated in the Cis-Ural region for infection with barley yellow dwarf virus (BYDV) and wheat streak mosaic virus (WSMV) is presented in this study. The relevance of this research stems from the growing impact of viral infections on grain crop productivity and the need to identify resistant genotypes in the context of climate change and increasing phytosanitary pressure on agrocenoses. The aim of this work was to assess the prevalence of Barley yellow dwarf virus (BYDV) and Wheat streak mosaic virus (WSMV) in winter rye crops in the Cis-Urals region. To achieve this, we used reverse transcription followed by real-time polymerase chain reaction (RT-PCR). The objects of study comprised eight domestically bred cultivars grown in the Cis-Ural region. It was established that BYDV is the dominant pathogen, detected in the majority of the studied cultivars, whereas WSMV exhibits a lower incidence rate. Significant inter-cultivar differences in infection rates were revealed, indirectly indicating genetically determined resistance. Certain cultivars demonstrated reduced susceptibility, positioning them as promising source material for further breeding. The scientific novelty of the work lies in obtaining new data on the prevalence of viral infections in rye within the Cis-Ural region using highly sensitive diagnostic methods. Practical significance of the research is attributed to the potential application of the results in developing phytosanitary monitoring strategies and selecting resistant cultivars. Future research prospects involve expanding the range of analyzed pathogens and investigating the molecular mechanisms of plant resistance.

KEYWORDS

winter rye, Secale cereale L., plant viral diseases, BYDV, WSMV, real-time PCR, cultivar resistance, phytosanitary monitoring

Funding

The research was carried out under the support of the Ministry of Science and Higher Education of the Russian Federation within the state assignment of the Perm Federal Research Center of the Ural Branch of the Russian Academy of Sciences (theme No. 122032200247-7).

1. Artemyeva, T.A., & Karpukhin, M.Yu. (2019). Comparison of ELISA and PCR analysis methods for detecting viral pathology in potato plants. Youth and Science. (78). 35. (in Russ.)

2. Batalova, G.A. (2017). Breeding of grain crops for immunity in the northeast of the European territory of Russia. Grain Economy of Russia. 3(51). 8-11. (in Russ.)

3. Bogoutdinov, D Z., Kastaleva, T.B., & Girsova, N.V. (2017). Viral diseases of grain crops in Samara Oblast: Bulletin of Orenburg State University. 4(204). 46-52. (in Russ.)

4. Kibkalo, I.A., & Safonova, I.V. (2026). Study of modern winter rye varieties for grain technological properties. Proceedings on Applied Botany, Genetics and Breeding. 187(1). 98-110. (in Russ.) DOI: https://doi.org/10.30901/2227-8834-2026-1-o-22

5. Nechaeva, Yu.S., Beltyukova, N.N., Prishnivskaya, Ya.V., & Taiman, K.E. (2011). Optimization of DNA extraction methods for some coniferous plant species of Perm Krai. In Proceedings of the international conference “Synthesis of knowledge in natural sciences: The Mine of the Future: projects, technologies, equipment”. 278-282. (in Russ.)

6. Ovesnov, S.A. (1997). Synopsis of the flora of Perm Oblast. Perm: Perm State National Research University. 252. (in Russ.)

7. Parfenova, E.S., & Psareva, E.A. (2024). Adaptability of winter rye varieties for grain yield in the conditions of Kirov Oblast. Agricultural Science of Euro-North-East. 25(4). 561-570. (in Russ.) https://doi.org/10.30766/2072-9081.2024.25.4.561–570 DOI: https://doi.org/10.30766/2072-9081.2024.25.4.561-570

8. Prikhodko, Yu.N., Zhivaeva, T.S., Lozovaya, E.N., Bashkirova, I.G., Karimova, E.V., Kalashnikov, A.A., & Shneyder, Yu.A. (2025). Wheat streak mosaic virus (Tritimovirus tritici) – prevalence on wheat in the Russian Federation and genetic characteristics of the revealed isolates. Grain Economy of Russia. 17(2). 94-102. (in Russ.). https://doi.org/10.31367/2079-8725-2025-97-2-94-102

9. Rybas, I.A. (2016). Increasing adaptability in grain crop breeding: Agricultural Biology. 51(5). 617-626. (in Russ.)

10. Chugaeva, V.V., Budko, A.S., & Karpovich, O.N. (2025). Economic and biological characteristics of a new winter tetraploid rye variety Zalesnaya. Bulletin of the Belarusian State Agricultural Academy. (2). 47-50. (in Russ.)

11. Sheshegova, T.K., Shchekleina, L.M., & Utkina, E.I. (2018). Immunological characterization of winter rye varieties. Agricultural Science of Euro-North-East. (4). 30-35. (in Russ.) DOI: https://doi.org/10.30766/2072-9081.2018.65.4.30-35

12. Yarkova, T.M., & Mashkin, V.S. (2023). Analysis and improvement of winter rye grain production efficiency in the Perm region. Scientific aspect. 9(12). 1050-1055. (in Russ.)

13. Devi, B.M., Guruprasath, S., Balu, P., Chattopadhyay, A., Thilagar, S.S., Dhanabalan, K.V., Choudhary, M.. Moparthi, S.. Jailani, A.A.K. (2024) Dissecting Diagnostic and Management Strategies for Plant Viral Diseases. What Next?: Agriculture. 14(2). 284. https://doi.org/10.1007/s00284-025-04280-4

14. Hafeez, A., Ali, B., Javed, M.A., Saleem, A., Fatima, M., Fathi, A., ... & Soudy, F.A. (2023). Plant breeding for harmony between sustainable agriculture, the environment, and global food security: an era of genomics‐assisted breeding. Planta. 258(5). 97. https://doi.org/10.1007/s00425-023-04252-7

15. Khorina, N.A., Lopatkin, A.A., Zhivaeva, T.S., Prikhodko, Y.N., & Shneyder, Y.A. (2023). Barley stripe mosaic virus–a serious pathogen affecting the export potential of the Russian Federation. Plant H. 1. 32-46.

16. Kim, N.K., Lee, H.J., Kim, S.M., & Jeong, R.D. (2022). Incidence and distribution of Barley yellow dwarf virus infecting oats in Korea. Research in Plant Disease. 28(1). 32-38. https://doi.org/10.5423/RPD.2022.28.1.32

17. Pomortsev, A.V., Dorofeev, N.V., Zorina, S.Y., Katysheva, N.B., Sokolova, L.G., Zhuravkova, A.S., & Mikhailova, E.V. (2023). Evaluation of population and hybrid varieties of winter rye in the conditions of Eastern Siberia. Agronomy. 13(5). 1431. https://doi.org/10.3390/agronomy13051431

18. Pozhylov, I., Snihur, H., Shevchenko, T., Budzanivska, I., Liu, W., Wang, X., & Shevchenko, O. (2022). Occurrence and characterization of wheat streak mosaic virus found in mono-and mixed infection with high plains wheat mosaic virus in winter wheat in Ukraine. Viruses. 14(6). 1220. https://doi.org/10.3390/v14061220

19. Rogers, S.O., & Bendich, A.J. (1985). Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant molecular biology. 5(2). 69-76. DOI: https://doi.org/10.1007/BF00020088

20. Roy, S.D., Ramasamy, S., & Obbineni, J.M. (2024). An evaluation of nucleic acid-based molecular methods for the detection of plant viruses: a systematic review. Virus Disease. 35(2). 357-376. https://doi.org/10.1007/s13337-024-00863-0

21. Tanu, Singh, V.K., Pandey, A., Gahlaut, V., & Kumar, A. (2025). Viral Challenges in Wheat: Comprehensive Diagnosis and Innovative Management Approaches. Current Microbiology. 82(7). 294. https://doi.org/10.1007/s00284-025-04280-4

22. Review of the phytosanitary condition of agricultural crops in the Perm Territory in 2023 // Federal State Budgetary Institution “Russian Agricultural Center” (FSBI “Rosselkhoztsentr”). URL: https://clck.ru/3UDXuR (reference’s date: 02.03.2026).

23. Review of the phytosanitary condition of agricultural crops in the Perm Territory in 2024 //Federal State Budgetary Institution “Russian Agricultural Center” (FSBI “Rosselkhoztsentr”). URL: https://clck.ru/3UDXto (reference’s date: 02.03.2026).

24. Soil map of the Russian Federation. URL: https://soil-db.ru/map (accessed: 29.03.2026)

This work is licensed under Creative Commons Attribution 4.0 International

JATS XML

This work is licensed under Creative Commons Attribution 4.0 International Creative Commons

GOST

Pechenkina V. ECOLOGICAL-GENETIC ANALYSIS OF SECALE CEREALE L. CULTIVARS GROWN IN CIS-URALS // . 2026. pp. 98-108. DOI: https://doi.org/10.36906/2311-4444/26-2/09. (Date of access 19.08.2026).

MLA

Viktoriya A. Pechenkina. "ECOLOGICAL-GENETIC ANALYSIS OF SECALE CEREALE L. CULTIVARS GROWN IN CIS-URALS." (2026): 98-108. DOI: https://doi.org/10.36906/2311-4444/26-2/09.

APA

Pechenkina V. (2026). ECOLOGICAL-GENETIC ANALYSIS OF SECALE CEREALE L. CULTIVARS GROWN IN CIS-URALS. , 98-108. https://doi.org/10.36906/2311-4444/26-2/09

Chicago

Viktoriya A. Pechenkina. "ECOLOGICAL-GENETIC ANALYSIS OF SECALE CEREALE L. CULTIVARS GROWN IN CIS-URALS." , no. (2026): 98-108. https://doi.org/10.36906/2311-4444/26-2/09.

WIKI

{{cite journal | author = Pechenkina V. | title = ECOLOGICAL-GENETIC ANALYSIS OF SECALE CEREALE L. CULTIVARS GROWN IN CIS-URALS | journal = | year = 2026 | pages = 98-108 | doi = 10.36906/2311-4444/26-2/09 }}

Copy

Export: BibTeX EndNote RefMan RusMARC

ECOLOGICAL-GENETIC ANALYSIS OF SECALE CEREALE L. CULTIVARS GROWN IN CIS-URALS

Confirmation